caa a22 4500 469090804 CHVBK 20180323133016.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF00419667 doi (NATIONALLICENCE)springer-10.1007/BF00419667 Refined definition of the 56K and other autoantigens in the 50-60 kDa region [Elektronische Daten] [G. Pruijn, B. Božič, F. Schoute, L. Rokeach, W. van Venrooij] Alteration of the acrylamide: bisacrylamide ratio in the SDS-polyacrylamide gel used for Western blotting strongly improved the unambiguous detection of antibodies against 50-60 kDa autoantigens present in autoimmune patient sera. The relative migration of Ro 52, the 56K autoantigen and calreticulin increased with reduced acrylamide: bisacrylamide ratios in contrast to that of Ro60, La and Jo-1. These analyses indicated that these six autoantigens correspond to six distinct polypeptides. Further analyses using recombinant calreticulin showed that (i) the 56K autoantigen is neither identical nor related to calreticulin and (ii) calreticulin is not a Ro autoantigen. A series of experiments designed to better characterize the 56K autoantigen showed that (i) the antigen is not detectable in fixed cells, presumably due to masking of the epitopes; (ii) about equal amounts of the antigen were recovered in nuclear and cytoplasmic cell, fractions after enucleation of the cells; (iii) the 56K autoantigen is not stably associated with either RNA or other proteins. Kluwer Academic Publishers, 1992 56K autoantigen nationallicence Autoimmunity nationallicence Calreticulin nationallicence Immunoblotting nationallicence Sjögren's syndrome nationallicence Systemic Lupus erythematosus nationallicence a- : anti- nationallicence CaR : calreticulin nationallicence NHS : normal human serum nationallicence NRS : normal rabbit serum nationallicence r : recombinant nationallicence RA : rheumatoid arthritis nationallicence SLE : systemic lupus erythematosus nationallicence SS : Sjögren's syndrome nationallicence Pruijn G. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, NL 6500 HB, Nijmegen, The Netherlands aut Božič B. Department of Rheumatology, University Medical Centre, Ljubljana, Slovenia aut Schoute F. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, NL 6500 HB, Nijmegen, The Netherlands aut Rokeach L. Department de Biochimie, Université de Montréal, Montréal, Canada aut van Venrooij W. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, NL 6500 HB, Nijmegen, The Netherlands aut Molecular Biology Reports Kluwer Academic Publishers 16/4(1992-09-01), 267-276 0301-4851 16:4<267 1992 16 11033 https://doi.org/10.1007/BF00419667 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00419667 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pruijn G. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, NL 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Božič B. Department of Rheumatology, University Medical Centre, Ljubljana, Slovenia aut NATIONALLICENCE 700 1- Schoute F. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, NL 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Rokeach L. Department de Biochimie, Université de Montréal, Montréal, Canada aut NATIONALLICENCE 700 1- van Venrooij W. Department of Biochemistry, University of Nijmegen, P.O. Box 9101, NL 6500 HB, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/4(1992-09-01), 267-276 0301-4851 16:4<267 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer