caa a22 4500 46909088X CHVBK 20180323133017.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00419757 doi (NATIONALLICENCE)springer-10.1007/BF00419757 Analysis of an immunodominant epitope of topoisomerase I in patients with systemic sclerosis [Elektronische Daten] [T. Meesters, M. Hoet, F. van den Hoogen, R. Verheijen, W. Habets, W. van Venrooij] Summary: In this paper an immunodominant epitope of Topoisomerase I is described. An epitope expression sublibrary was constructed from Topoisomerase I cDNA. The subclones were screened with an antiserum from a patient with systemic sclerosis (SSc). The positive clones defined one immunodominant B cell epitope (epitope III), which was located at the carboxyterminal part of the protein. The epitope, 52 amino acids in length, neither contains the p 30gag sequence nor the suggested active site Tyr-723, both presumed antibody recognition sites. More than 70% of our anti-TopoI sera recognize this epitope III, indicating that it is a major recognition site of the anti-TopoI autoantibodies in SSc sera. DNA relaxation experiments show that all sera that recognize epitope III and most sera with antibodies to other epitopes inhibit Topoisomerase I activity. Kluwer Academic Publishers, 1992 autoimmunity nationallicence connective tissue disease nationallicence B cell epitope nationallicence Topoisomerase I nationallicence autoantibodies nationallicence autoantigen nationallicence SSc : Systemic sclerosis nationallicence Topol : DNA Topoisomerase I (EC 5.99.1.2) nationallicence Meesters T. Department of Biochemistry, University of Nijmegen, The Netherlands aut Hoet M. Department of Biochemistry, University of Nijmegen, The Netherlands aut van den Hoogen F. Department of Rheumatology, University Hospital Nijmegen, The Netherlands aut Verheijen R. Department of Biochemistry, University of Nijmegen, The Netherlands aut Habets W. Department of Biochemistry, University of Nijmegen, The Netherlands aut van Venrooij W. Department of Biochemistry, University of Nijmegen, The Netherlands aut Molecular Biology Reports Kluwer Academic Publishers 16/2(1992-05-01), 117-123 0301-4851 16:2<117 1992 16 11033 https://doi.org/10.1007/BF00419757 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00419757 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Meesters T. Department of Biochemistry, University of Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Hoet M. Department of Biochemistry, University of Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- van den Hoogen F. Department of Rheumatology, University Hospital Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Verheijen R. Department of Biochemistry, University of Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Habets W. Department of Biochemistry, University of Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- van Venrooij W. Department of Biochemistry, University of Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/2(1992-05-01), 117-123 0301-4851 16:2<117 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer