caa a22 4500 469090901 CHVBK 20180323133017.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00419755 doi (NATIONALLICENCE)springer-10.1007/BF00419755 Deletion analysis of the NH2-terminal region of β-1,4-galactosyltransferase [Elektronische Daten] [Arni Masibay, Elisabeth Boeggeman, Pradman Qasba] To determine the biological role, if any, of the NH2-terminal region of β-1,4-galactosyltransferase (GT; EC 2.4.1.90), we constructed deletion mutants and expressed them in COS-7 cells. Each deletion construct was analyzed for enzymatic activity, protein production and mRNA transcription. All of the deletion mutants were transcribed to produce GT mRNA, but the GT protein was not detected in those constructs whose transmembrane (aa 14-42) domain was deleted. The results suggest that the transmembrane region is essential for the stability of the protein and perhaps contain sequences critical for the proper targeting of the molecule. The possible role of the NH2-terminal signal anchor domain in the in vivo regulation of GT is discussed. Kluwer Academic Publishers, 1992 glycosyltransferase nationallicence expression nationallicence structure/function nationallicence aa : amino acid nationallicence GT : β-1,4-Galactosyltransferase nationallicence GlcNAc : N-AcetylGlucosamine nationallicence TM : transmembrane nationallicence Masibay Arni Laboratory of Mathematical Biology; Division of Cancer Biology, Diagnosis, and Centers; National Cancer Institute, National Institutes of Health, 20892, Bethesda, MD, USA aut Boeggeman Elisabeth Laboratory of Mathematical Biology; Division of Cancer Biology, Diagnosis, and Centers; National Cancer Institute, National Institutes of Health, 20892, Bethesda, MD, USA aut Qasba Pradman Laboratory of Mathematical Biology; Division of Cancer Biology, Diagnosis, and Centers; National Cancer Institute, National Institutes of Health, 20892, Bethesda, MD, USA aut Molecular Biology Reports Kluwer Academic Publishers 16/2(1992-05-01), 99-104 0301-4851 16:2<99 1992 16 11033 https://doi.org/10.1007/BF00419755 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00419755 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Masibay Arni Laboratory of Mathematical Biology; Division of Cancer Biology, Diagnosis, and Centers; National Cancer Institute, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Boeggeman Elisabeth Laboratory of Mathematical Biology; Division of Cancer Biology, Diagnosis, and Centers; National Cancer Institute, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Qasba Pradman Laboratory of Mathematical Biology; Division of Cancer Biology, Diagnosis, and Centers; National Cancer Institute, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 773 0- Molecular Biology Reports Kluwer Academic Publishers 16/2(1992-05-01), 99-104 0301-4851 16:2<99 1992 16 11033 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer