caa a22 4500 469092734 CHVBK 20180323133022.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00572125 doi (NATIONALLICENCE)springer-10.1007/BF00572125 The application of whole-cell protein electrophoresis for the classification and identification of basidiomycetous yeast species [Elektronische Daten] [Marc Vancanneyt, Eddy Van Lerberge, Jean-Francois Berny, Gregoire Hennebert, Karel Kersters] The relationships among 65 basidiomycetous yeast strains were determined by one-dimensional electrophoresis of SDS-solubilized whole-cell proteins. Protein profiles were compared by the Pearson product moment correlation coefficient (r). The strains investigated represented species from the generaCystofilobasidium, Filobasidium, Filobasidiella, Kondoa, Leucosporidium, Mrakia andRhodosporidium. Except for the genusMrakia, all species constituted separate protein electrophoretic clusters. The species of the genusMrakia (M. frigida, M. gelida, M. nivalis andM. stokesii) show highly similar protein patterns, suggesting that these four species may be synonymous. Strains of two varieties ofFilobasidiella neoformans, F. neoformans var.neoformans andF. neoformans var.bacillispora, could not be differentiated by protein electrophoresis. For the delineation of the protein electrophoretic clusters of the yeasts studied, literature data relying on other criteria, such as DNA base composition, carbon source utilization patterns, enzymatic protein electrophoregrams, ubiquinone systems, DNA-DNA homology and rRNA sequence data were used. It was demonstrated that a database of SDS-protein patterns provides a valuable tool for the identification of yeasts. Kluwer Academic Publishers, 1992 basidiomycetous yeasts nationallicence protein electrophoregrams nationallicence identification nationallicence taxonomy nationallicence Vancanneyt Marc Laboratorium voor Microbiologie en microbiële Genetica, Rijksuniversiteit Gent, 9000, Gent, Belgium aut Van Lerberge Eddy Laboratorium voor Microbiologie en microbiële Genetica, Rijksuniversiteit Gent, 9000, Gent, Belgium aut Berny Jean-Francois Mycothèque de l'Université Catholique de Louvain, 1348, Louvain-la-Neuve, Belgium aut Hennebert Gregoire Mycothèque de l'Université Catholique de Louvain, 1348, Louvain-la-Neuve, Belgium aut Kersters Karel Laboratorium voor Microbiologie en microbiële Genetica, Rijksuniversiteit Gent, 9000, Gent, Belgium aut Antonie van Leeuwenhoek Kluwer Academic Publishers 61/1(1992-01-01), 69-78 0003-6072 61:1<69 1992 61 10482 https://doi.org/10.1007/BF00572125 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00572125 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vancanneyt Marc Laboratorium voor Microbiologie en microbiële Genetica, Rijksuniversiteit Gent, 9000, Gent, Belgium aut NATIONALLICENCE 700 1- Van Lerberge Eddy Laboratorium voor Microbiologie en microbiële Genetica, Rijksuniversiteit Gent, 9000, Gent, Belgium aut NATIONALLICENCE 700 1- Berny Jean-Francois Mycothèque de l'Université Catholique de Louvain, 1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Hennebert Gregoire Mycothèque de l'Université Catholique de Louvain, 1348, Louvain-la-Neuve, Belgium aut NATIONALLICENCE 700 1- Kersters Karel Laboratorium voor Microbiologie en microbiële Genetica, Rijksuniversiteit Gent, 9000, Gent, Belgium aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 61/1(1992-01-01), 69-78 0003-6072 61:1<69 1992 61 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer