caa a22 4500 469092777 CHVBK 20180323133022.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00572120 doi (NATIONALLICENCE)springer-10.1007/BF00572120 Purification and properties of an extra cellular xylanase enzyme of Clostridium strain SAIV [Elektronische Daten] [M. Murty, T. Chandra] An extracellular xylanase enzyme fraction A from a mesophilicClostridium strain SAIV was purified by ammonium sulfate precipitation, Sephadex G-50 gel filtration and DEAE-Sephadex A-50 ion exchange. The xylanase exhibited a molecular weight of ≁30,000 and it was stable upto 55° C with an optimum temperature of 50° C. It was most stable between pH 5-7, with an optimum pH of around 6. The Km value was 7.0 mg·xylan ml-1 and Vmax was 36 μmol·xylose liberated mg-1 min-1. Carboxymethyl cellulose, filter paper cellulose and 4-p-nitrophenyl β-D-xylopyranoside were not hydrolysed. The specific activity of xylanase fraction A (9.8 U mg-1) is 2-10 fold higher than the specific activity of xylanase in other mesophilic, xylanolytic, obligate anaerobic bacteria. A minor fraction of xylanase activity designated as xylanase B was also obtained supporting the view that the multiplicity of xylanases is common in microorganisms. Kluwer Academic Publishers, 1992 anaerobes nationallicence Clostridium nationallicence xylanase nationallicence xylan nationallicence Murty M. Department of Chemistry, Biochemistry Division, Indian Institute of Technology, 600 036, Madras, India aut Chandra T. Department of Chemistry, Biochemistry Division, Indian Institute of Technology, 600 036, Madras, India aut Antonie van Leeuwenhoek Kluwer Academic Publishers 61/1(1992-01-01), 35-41 0003-6072 61:1<35 1992 61 10482 https://doi.org/10.1007/BF00572120 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00572120 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Murty M. Department of Chemistry, Biochemistry Division, Indian Institute of Technology, 600 036, Madras, India aut NATIONALLICENCE 700 1- Chandra T. Department of Chemistry, Biochemistry Division, Indian Institute of Technology, 600 036, Madras, India aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 61/1(1992-01-01), 35-41 0003-6072 61:1<35 1992 61 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer