caa a22 4500 469092785 CHVBK 20180323133022.0 cr unu---uuuuu 170328e19920101xx s 000 0 eng 10.1007/BF00572124 doi (NATIONALLICENCE)springer-10.1007/BF00572124 Zevenhuizen L. Laboratory of Microbiology, Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT, Wageningen, The Netherlands aut Levels of trehalose and glycogen in Arthrobacter globiformis under conditions of nutrient starvation and osmotic stress [Elektronische Daten] [L. Zevenhuizen] Cells ofArthrobacter globiformis grown in carbohydrate-rich media were found to contain large quantities of low-Mr carbohydrates (800 μg/mg protein) and only small amounts of amino acids, in addition to high amounts of glycogen (2 mg/mg protein). At increasing osmotic values of the medium, low-Mr carbohydrate levels increased to 1300 μg/mg protein. Low-Mr pools were extracted from the cells with hot 75% ethanol, and subjected to thin layer, gel and gas-liquid chromatography. They turned out to consist mainly of α,α-trehalose. Levels of trehalose inArthrobacter cells have the tendency to remain constant, both during nutrient exhaustion (resulting in glycogen consumption), and on addition of excess of carbon source to the medium (resulting in an increased glycogen content of the cells). The stress-tolerant properties ofArthrobacter (resistance to nutrient starvation, desiccation and high salt concentration) are discussed with respect to the high glycogen and trehalose contents of the cells. Kluwer Academic Publishers, 1992 Arthrobacter nationallicence glycogen nationallicence osmoregulation nationallicence trehalose nationallicence Antonie van Leeuwenhoek Kluwer Academic Publishers 61/1(1992-01-01), 61-68 0003-6072 61:1<61 1992 61 10482 https://doi.org/10.1007/BF00572124 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00572124 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Zevenhuizen L. Laboratory of Microbiology, Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 61/1(1992-01-01), 61-68 0003-6072 61:1<61 1992 61 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer