caa a22 4500 469092866 CHVBK 20180323133022.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00584225 doi (NATIONALLICENCE)springer-10.1007/BF00584225 Genetic analysis of methylotrophic yeast Candida boidinii PLD1 [Elektronische Daten] [Kantcho Lahtchev, Ruminia Penkova, Veselina Ivanova, Dimka Tuneva] This paper reports the initial experiments for genetic analysis of the haploid methylotrophic yeastCandida boidinii PLD1. The collection of multiply marked auxotrophic mutants was obtained after treatment with UV-light or X-rays. Protoplasts from several mutants were fused by the PEG-CA2+ technique and five prototrophic hybrids were isolated. The genetic structure of the hybrids was studied by means of spontaneous and induced mitotic segregation. Our data suggest that hybrids are diploids, heterozygous by parental auxotrophic markers. We obtained genetic linkage between mutationslys2-8-met-3 from one hand andade-17-arg-24 from the other. The genetic maps constructed showed similar characteristics concerning both the order of the markers and their map distances. Kluwer Academic Publishers, 1992 methylotrophic yeasts nationallicence protoplast fusion nationallicence mitotic segregation nationallicence genetic mapping nationallicence Candida boidinii nationallicence Lahtchev Kantcho Institute of Microbiology, Bulgarian Academy of Science, Sofia, Bulgaria aut Penkova Ruminia Institute of Microbiology, Bulgarian Academy of Science, Sofia, Bulgaria aut Ivanova Veselina Institute of Microbiology, Bulgarian Academy of Science, Sofia, Bulgaria aut Tuneva Dimka Institute of Microbiology, Bulgarian Academy of Science, Sofia, Bulgaria aut Antonie van Leeuwenhoek Kluwer Academic Publishers 61/3(1992-04-01), 185-194 0003-6072 61:3<185 1992 61 10482 https://doi.org/10.1007/BF00584225 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00584225 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lahtchev Kantcho Institute of Microbiology, Bulgarian Academy of Science, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Penkova Ruminia Institute of Microbiology, Bulgarian Academy of Science, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Ivanova Veselina Institute of Microbiology, Bulgarian Academy of Science, Sofia, Bulgaria aut NATIONALLICENCE 700 1- Tuneva Dimka Institute of Microbiology, Bulgarian Academy of Science, Sofia, Bulgaria aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 61/3(1992-04-01), 185-194 0003-6072 61:3<185 1992 61 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer