caa a22 4500 469092874 CHVBK 20180323133022.0 cr unu---uuuuu 170328e19920401xx s 000 0 eng 10.1007/BF00584226 doi (NATIONALLICENCE)springer-10.1007/BF00584226 Physical and genetic characterization of linear DNA plasmids from the heterothallic yeast Saccharomycopsis crataegensis [Elektronische Daten] [P. Bolen, C. Kurtzman, J. Ligon, B. Mannarelli, R. Bothast] Five strains of the heterothallic yeastSaccharomycopsis crataegensis have been previously shown to contain DNA and/or RNA plasmidlike molecules (Shepherd et al. 1987). Three DNA plasmids, designated pScrl-1,-2 and -3, were found in strain NRRL Y-5902, while two were identified in each of NRRL strains Y-5903 and Y-5904. DNA plasmids were not identified inS. crataegensis strains Y-5910 or YB-192. FourS. crataegensis strains (Y-5903, Y-5904, Y-5910 and YB-192) were also shown to possess double-stranded RNA (dsRNA) molecules not found in strain Y-5902 (Shepherd et al. 1987). Hybridization studies now demonstrate the DNA plasmids in Y-5903 and Y-5904 to be highly homologous to their respective size counterparts (pScrl-1 and pScrl-2) in Y-5902 and to show some homology to pScrl-3. Restriction endonuclease mapping studies confirm the linear nature of each plasmid and establish identical restriction maps for a 1.4 kilobase (kb) region in pScrl-2 and -3. This 1.4 kb region accounts for the hybridization homology of pScrl-2 and pScrl-3 noted by Shepherd et al. (1987) and for homology of the plasmids of Y-5903 and Y-5904 to pScrl-3 of Y-5902. The pScrl plasmids show no homology to the dsRNA molecules ofS. crataegensis, the 2 μM circular DNA ofStaccharomyces cerevisiae, the ‘killer' plasmids ofKluyveromyces lactis, or the linear DNA plasmids ofPichia inositovora. In crosses between linear DNA plasmid-containing and dsRNA-containing strains, only progeny containing the pScrl plasmids were recovered. Poor spore viability and a lack of complete tetrad recovery limited the extent of the analysis, but the findings suggest a cytoplasmic mode of inheritance for these linear DNAs. Kluwer Academic Publishers, 1992 fungi nationallicence Saccharomycopsis crataegensis nationallicence yeast nationallicence plasmid nationallicence linear DNA nationallicence p Scr l-1 nationallicence p Scr l-2 p Scr l-3 nationallicence curing nationallicence Bolen P. National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA aut Kurtzman C. National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA aut Ligon J. National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA aut Mannarelli B. National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA aut Bothast R. National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA aut Antonie van Leeuwenhoek Kluwer Academic Publishers 61/3(1992-04-01), 195-205 0003-6072 61:3<195 1992 61 10482 https://doi.org/10.1007/BF00584226 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00584226 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bolen P. National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA aut NATIONALLICENCE 700 1- Kurtzman C. National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA aut NATIONALLICENCE 700 1- Ligon J. National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA aut NATIONALLICENCE 700 1- Mannarelli B. National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA aut NATIONALLICENCE 700 1- Bothast R. National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 61604, Peoria, Illinois, USA aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 61/3(1992-04-01), 195-205 0003-6072 61:3<195 1992 61 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer