caa a22 4500 469092882 CHVBK 20180323133022.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00713933 doi (NATIONALLICENCE)springer-10.1007/BF00713933 Arginine and proline genes of Aspergillus niger [Elektronische Daten] [Klaas Swart, Alfons Debets, Gerda Kobus, Cees Bos] Summary: Aspergillus niger mutants defective in arginine or proline biosynthesis have been isolated and 12 genetic loci were identified. Mutation was induced by low doses UV, and mutants were isolated after filtration enrichment. The mutants were classified according to their phenotype in growth tests and were further characterized in complementation tests. The arginine auxotrophic mutants represent nine complementation groups. Three additional complementation groups were found for mutants that could grow on proline (two of them on arginine too). Linkage group analysis was done in somatic diploids obtained from a mutant and a master strain with genetic markers on six chromosomes. Thearg genes belong to six different linkage groups and thepro genes to two. Onearg-mutant could be complemented by transformation with theA. nidulans arg B + gene, and thisA. niger gene thus appeared to be homologous to theA. nidulans arg B. We isolated anA. niger strain with theargB gene tightly linked with thenicA1 marker. This strain is very suitable as acceptor for transformation with anargB-plasmid, because transformants with inserts on the homologous site can be recognized and analyzed genetically using thenicA1 marker gene. Kluwer Academic Publishers, 1992 arginine mutants nationallicence Aspergillus niger nationallicence proline mutants nationallicence linkage groups nationallicence somatic recombination nationallicence Swart Klaas Department of Genetics, Agricultural University, Dreyenlaan 2, 6703 HA, Wageningen, The Netherlands aut Debets Alfons Department of Genetics, Agricultural University, Dreyenlaan 2, 6703 HA, Wageningen, The Netherlands aut Kobus Gerda Department of Genetics, Agricultural University, Dreyenlaan 2, 6703 HA, Wageningen, The Netherlands aut Bos Cees Department of Genetics, Agricultural University, Dreyenlaan 2, 6703 HA, Wageningen, The Netherlands aut Antonie van Leeuwenhoek Kluwer Academic Publishers 61/4(1992-05-01), 259-264 0003-6072 61:4<259 1992 61 10482 https://doi.org/10.1007/BF00713933 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00713933 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Swart Klaas Department of Genetics, Agricultural University, Dreyenlaan 2, 6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Debets Alfons Department of Genetics, Agricultural University, Dreyenlaan 2, 6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Kobus Gerda Department of Genetics, Agricultural University, Dreyenlaan 2, 6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Bos Cees Department of Genetics, Agricultural University, Dreyenlaan 2, 6703 HA, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 61/4(1992-05-01), 259-264 0003-6072 61:4<259 1992 61 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer