caa a22 4500 469092904 CHVBK 20180323133022.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00713932 doi (NATIONALLICENCE)springer-10.1007/BF00713932 The value of electrophoretic fingerprinting and karyotyping in wine yeast breeding programmes [Elektronische Daten] [T. van der Westhuizen, I. Pretorius] Electrophoretic banding pattens of total soluble cell proteins, DNA restriction fragments and chromosomal DNA were used to characterise ten strains ofSaccharomyces cerevisiae used for commercial production of wine. These fingerprinting procedures provided unique profiles for all the different yeast strains and can therefore be used to identify and control industrial strains. Furthermore, the protein profiles, restriction fragments banding patterns and electrophoretic karyotyping by contour clamped homogeneous electric field electrophoresis (CHEF), were valuable to differentiate hybrid and parental strains in yeast breeding programmes. Hybrid strains, with desirable oenological properties, were obtained by mass spore-cell mating between a heterothallic killer yeast and two homothallic sensitive strains and all were shown to have unique DNA fingerprints and electrophoretic karyotypes. Kluwer Academic Publishers, 1992 wine yeasts nationallicence fingerprinting nationallicence CHEF nationallicence karyotyping nationallicence spore-cell mating nationallicence van der Westhuizen T. Department of Microbiology and Institute for Biotechnology, University of Stellenbosch, 7600, Stellenbosch, South Africa aut Pretorius I. Department of Microbiology and Institute for Biotechnology, University of Stellenbosch, 7600, Stellenbosch, South Africa aut Antonie van Leeuwenhoek Kluwer Academic Publishers 61/4(1992-05-01), 249-257 0003-6072 61:4<249 1992 61 10482 https://doi.org/10.1007/BF00713932 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00713932 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van der Westhuizen T. Department of Microbiology and Institute for Biotechnology, University of Stellenbosch, 7600, Stellenbosch, South Africa aut NATIONALLICENCE 700 1- Pretorius I. Department of Microbiology and Institute for Biotechnology, University of Stellenbosch, 7600, Stellenbosch, South Africa aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 61/4(1992-05-01), 249-257 0003-6072 61:4<249 1992 61 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer