caa a22 4500 469092947 CHVBK 20180323133022.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00713940 doi (NATIONALLICENCE)springer-10.1007/BF00713940 Detection of Aspergillus and Penicillium extracellular polysaccharides (EPS) by ELISA: using antibodies raised against acid hydrolysed EPS [Elektronische Daten] [Henri Kamphuis, Gerhard De Ruiter, Gerrit Veeneman, Jacques van Boom, Frank Rombouts, Servé Notermans] Species of the fungal generaAspergillus andPenicillium produce immunologically active extracellular polysaccharides (EPS) in which galactofuranose residues are immunodominant. The antigenic determinant of the EPSA. fumigatus, A. niger andP. digitatum could be removed by acid hydrolysis. Due to the hydrolysis of the EPS the immunological reaction between IgG anti-native EPS and hydrolysed EPS disappeared. Antibodies raised in rabbits against the acid hydrolysed EPS revealed new antigenic determinants that were exposed as a result of the acid hydrolysis. Immunological inhibitory experiments showed that the antibodies were no longer directed to galactofuranose residues. Enzyme Linked Immunosorbent Assay, carried out with antibodies raised against the acid hydrolysed EPS showed that the antibodies against the acid hydrolysed EPS were more species specific in comparison with the antibodies against the native EPS. Kluwer Academic Publishers, 1992 extracellular polysaccharides nationallicence antibodies nationallicence immuno-assay nationallicence Aspergillus nationallicence Penicillium nationallicence Kamphuis Henri Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut De Ruiter Gerhard Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut Veeneman Gerrit Gorlaeus Laboratory, P.O. Box 9502, 2300 RA, Leiden, The Netherlands aut van Boom Jacques Gorlaeus Laboratory, P.O. Box 9502, 2300 RA, Leiden, The Netherlands aut Rombouts Frank Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut Notermans Servé National Institute of Public Health and Environmental Protection, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands aut Antonie van Leeuwenhoek Kluwer Academic Publishers 61/4(1992-05-01), 323-332 0003-6072 61:4<323 1992 61 10482 https://doi.org/10.1007/BF00713940 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00713940 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kamphuis Henri Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- De Ruiter Gerhard Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Veeneman Gerrit Gorlaeus Laboratory, P.O. Box 9502, 2300 RA, Leiden, The Netherlands aut NATIONALLICENCE 700 1- van Boom Jacques Gorlaeus Laboratory, P.O. Box 9502, 2300 RA, Leiden, The Netherlands aut NATIONALLICENCE 700 1- Rombouts Frank Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Notermans Servé National Institute of Public Health and Environmental Protection, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 61/4(1992-05-01), 323-332 0003-6072 61:4<323 1992 61 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer