caa a22 4500 469092955 CHVBK 20180323133022.0 cr unu---uuuuu 170328e19920501xx s 000 0 eng 10.1007/BF00713935 doi (NATIONALLICENCE)springer-10.1007/BF00713935 Alterations in the cell wall of Saccharomyces cerevisiae induced by the alpha sex factor or a mutation in the cell cycle [Elektronische Daten] [Silvia Díaz, Samuel Zínker, José Ruiz-Herrera] We performed experiments in parallel to study the rate of synthesis of cell wall polysaccharides and the activity of glycosyl transferases inSaccharomyces cerevisiae after arrest of acdc 28 mutant in G1 phase by either addition of alpha-factor or transfer to the non-permissive temperature. Both effectors brought about similar time-dependent increases in the rate of synthesis and deposition of the cell wall polysaccharides chitin, glucan and mannan. These changes in cell wall composition were accompanied by an increase in the specific activities of glucan and chitin synthetases. This increase was inhibited by cycloheximide suggesting that it representedde novo enzyme biosynthesis and not enzyme activation. Our data are consistent with the notion that both alpha-factor and thecdc 28 mutation affect the same stage-specific function that controls the temporal expression of glycosyl transferases. Kluwer Academic Publishers, 1992 Saccharomyces cerevisiae nationallicence cell wall nationallicence alpha-factor nationallicence cdc mutant nationallicence chitin nationallicence glucan nationallicence GlcNAc : N-acetyl glucosamine nationallicence UDPGIcNAc : uridine-diphosphate-N-acetyl glucosamine nationallicence UDPGlc : uridine-diphosphate glucose nationallicence TCA : trichloroacetic acid nationallicence EDTA : ethylene diamino tetraacetate nationallicence TAME : tosyl-L-arginyl methyl ester nationallicence GTP : guanosine triphosphate nationallicence WGA : wheat germ agglutinin nationallicence Díaz Silvia Departamento de Genética y Biología Molecular, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, México aut Zínker Samuel Departamento de Genética y Biología Molecular, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, México aut Ruiz-Herrera José Departamento de Genética y Biología Molecular, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, México aut Antonie van Leeuwenhoek Kluwer Academic Publishers 61/4(1992-05-01), 269-276 0003-6072 61:4<269 1992 61 10482 https://doi.org/10.1007/BF00713935 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00713935 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Díaz Silvia Departamento de Genética y Biología Molecular, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, México aut NATIONALLICENCE 700 1- Zínker Samuel Departamento de Genética y Biología Molecular, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, México aut NATIONALLICENCE 700 1- Ruiz-Herrera José Departamento de Genética y Biología Molecular, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, México aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 61/4(1992-05-01), 269-276 0003-6072 61:4<269 1992 61 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer