caa a22 4500 469093137 CHVBK 20180323133023.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00582577 doi (NATIONALLICENCE)springer-10.1007/BF00582577 Jahns Thomas 13.3 Fachrichtung Mikrobiologie der Universität des Saarlandes, Im Stadtwald, D-W-6600, Saarbrücken, Germany aut Regulation of urea uptake in Pseudomonas aeruginosa [Elektronische Daten] [Thomas Jahns] The energy-dependent urea permease was studied in two strains ofPseudomonas aeruginosa, measuring the uptake (transport and metabolism) of14C-urea. In both strains urea uptakein vivo and urease activityin vitro differed significantly with respect to kinetic parameters, temperature and pH dependence and response to metabolic inhibitors. Ammonium strongly interfered both with the expression of the urea uptake system and its activity. The inhibition of the uptake activity by ammonium was partially relieved by hydraziniumsulfate, which prevented the translocation of ammonium into the cell, and in a methylammonium/ammonium transport-defective mutant of strain DSM 50071. Furthermore, methionine-sulfoximine, which prevented the intracellular glutamine formation from ammoniumvia inhibition of glutamine synthetase, relieved the inhibition of urea uptake by ammonium. These findings suggested that urea uptake activity inP. aeruginosa is regulated by intracellular glutamine. Kluwer Academic Publishers, 1992 activity nationallicence Pseudomonas aeruginosa nationallicence regulation nationallicence urea uptake nationallicence urease nationallicence CCCP : carbonylcyanide-m-chlorphenylhydrazone nationallicence DCCD : dicyclohexylcarbodiimide nationallicence GS : glutamine synthetase nationallicence MSX : methionine-sulfoximine nationallicence Antonie van Leeuwenhoek Kluwer Academic Publishers 62/3(1992-10-01), 173-179 0003-6072 62:3<173 1992 62 10482 https://doi.org/10.1007/BF00582577 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00582577 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Jahns Thomas 13.3 Fachrichtung Mikrobiologie der Universität des Saarlandes, Im Stadtwald, D-W-6600, Saarbrücken, Germany aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 62/3(1992-10-01), 173-179 0003-6072 62:3<173 1992 62 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer