caa a22 4500 469093161 CHVBK 20180323133023.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00582579 doi (NATIONALLICENCE)springer-10.1007/BF00582579 Formation of antigenic extracellular polysaccharides by selected strains of Mucor spp., Rhizopus spp., Rhizomucor spp., Absidia corymbifera and Syncephalastrum racemosum [Elektronische Daten] [Gerhard De Ruiter, Andrea Van Bruggen-van der Lugt, M. Nout, Wouter Middelhoven, Pop Soentoro, Servé Notermans, Frank Rombouts] In this study, polyclonal IgG antibodies raised against extracellular polysaccharides (EPS) ofMucor racemosus were characterised as almost specific for moulds belonging to the order of Mucorales. Cross-reactivity in the ELISA could be observed only towards the yeastPichia membranaefaciens. EPS were isolated from various cultures ofM. hiemalis growing on six different carbon sources and two nitrogen sources, with ratios varying from 0.13 to 0.44 relative to the amount of biomass. Other strains includingMucor spp.,Rhizopus spp.,Rhizomucor spp.,Absidia corymbifera andSyncephalastrum racemosum also excreted EPS, with ratios varying from 0.05 to 0.23. In all cases, the excreted EPS had similar antigenic properties as determined by ELISA. No enzymatic degradation of the antigenic parts of the polysaccharides could be observed upon prolonged incubation. Considering that all tested strains formed similar amounts of antigenic EPS there might be scope for the specific detection of biomass of Mucoralean moulds using ELISA techniques for example in food. Kluwer Academic Publishers, 1992 antigen nationallicence ELISA nationallicence extracellular polysaccharides nationallicence Mucor nationallicence Pichia nationallicence Rhizopus nationallicence De Ruiter Gerhard Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut Van Bruggen-van der Lugt Andrea Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut Nout M. Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut Middelhoven Wouter Department of Microbiology, Wageningen Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT, Wageningen, The Netherlands aut Soentoro Pop Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Protection, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands aut Notermans Servé Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Protection, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands aut Rombouts Frank Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut Antonie van Leeuwenhoek Kluwer Academic Publishers 62/3(1992-10-01), 189-199 0003-6072 62:3<189 1992 62 10482 https://doi.org/10.1007/BF00582579 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00582579 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- De Ruiter Gerhard Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Van Bruggen-van der Lugt Andrea Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Nout M. Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Middelhoven Wouter Department of Microbiology, Wageningen Agricultural University, Hesselink van Suchtelenweg 4, 6703 CT, Wageningen, The Netherlands aut NATIONALLICENCE 700 1- Soentoro Pop Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Protection, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands aut NATIONALLICENCE 700 1- Notermans Servé Laboratory for Water and Food Microbiology, National Institute of Public Health and Environmental Protection, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands aut NATIONALLICENCE 700 1- Rombouts Frank Department of Food Science, Wageningen Agricultural University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 62/3(1992-10-01), 189-199 0003-6072 62:3<189 1992 62 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer