caa a22 4500 469093188 CHVBK 20180323133023.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00582576 doi (NATIONALLICENCE)springer-10.1007/BF00582576 Janatová Ivana Institute of Microbiology, Czechoslovak Academy of Sciences, Vídeňská 1083, 142 20, Prague 4, Czechoslovakia aut Isolation of auxotrophic mutants of the methylotrophic yeast Candida boidinii and determination of its ploidy [Elektronische Daten] [Ivana Janatová] Auxotrophic mutations in the methylotrophic yeast strainCandida boidinii 11Bh were induced by different mutagens and their combinations (nitrosoguanidine, UV light, HNO2+UV). Majority of the mutants obtained carried defects in histidine, arginine, proline and/or adenine biosynthetic pathways. His- mutants were distributed into four complementation groups using the protoplasts fusion technique. Ploidy determination ofCandida boidinii 11Bh was performed by measuring its DNA content and by following its survival after chemical mutagens treatment. The DNA content of this strain was found to be similar to that of aSaccharomyces cerevisiae diploid strain. Also the kinetics of survival ofCandida boidinii cells indicate thatCandida boidinii 11Bh is a diploid. Kluwer Academic Publishers, 1992 Candida boidinii nationallicence mutagenesis nationallicence auxotrophic mutant nationallicence ploidy nationallicence Antonie van Leeuwenhoek Kluwer Academic Publishers 62/3(1992-10-01), 167-171 0003-6072 62:3<167 1992 62 10482 https://doi.org/10.1007/BF00582576 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00582576 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Janatová Ivana Institute of Microbiology, Czechoslovak Academy of Sciences, Vídeňská 1083, 142 20, Prague 4, Czechoslovakia aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 62/3(1992-10-01), 167-171 0003-6072 62:3<167 1992 62 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer