caa a22 4500 46909320X CHVBK 20180323133023.0 cr unu---uuuuu 170328e19921001xx s 000 0 eng 10.1007/BF00582582 doi (NATIONALLICENCE)springer-10.1007/BF00582582 Genomic studies on killer yeasts belonging to the genus Pichia [Elektronische Daten] [Giulia Morace, Paola Cattani, Stefania Manzara, Stefania Conti, Mara Gerloni, Maurizio Sanguinetti, Brunella Posteraro, Luciano Polonelli] Twenty-four species belonging to the genusPichia were investigated using restriction fragment length polymorphism (RFLP) and Southern blot hybridization of their genomic DNA.Saccharomyces cerevisiae, Kluyveromyces lactis, Williopsis mrakii andCandida albicans were also included in this study. The RFLP patterns were obtained from digestion of yeast DNA with several restriction endonuclease enzymes, and showed various bands with different mobility; in most isolates, the more deeply stained bands were species-specific. This observation was confirmed by the results obtained from Southern blot hybridization of theEcoRI andXhoI RFLP patterns withP. anomala UCSC 25F DNA, digested with the same enzymes, used as probes. These bands are likely to be ribosomal DNA as shown by hybridization of digested DNA from unrelated yeast species (S. cerevisiae, K. lactis andC. albicans). However, one hybridized band, located at 3.9-4.1 Kb, seems to be peculiar to thePichia species. Our study confirms the usefulness of molecular tools in studying genetic relatedness among yeasts. Kluwer Academic Publishers, 1992 DNA fingerprinting nationallicence DNA-DNA hybridization nationallicence killer yeasts nationallicence Pichia spp nationallicence Morace Giulia Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo Francesco Vito, 1-00168, Roma, Italy aut Cattani Paola Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo Francesco Vito, 1-00168, Roma, Italy aut Manzara Stefania Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo Francesco Vito, 1-00168, Roma, Italy aut Conti Stefania Istituto di Microbiologia, Università degli Studi di Parma, Viale Gramsci, 14-43100, Italy aut Gerloni Mara Istituto di Microbiologia, Università degli Studi di Parma, Viale Gramsci, 14-43100, Italy aut Sanguinetti Maurizio Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo Francesco Vito, 1-00168, Roma, Italy aut Posteraro Brunella Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo Francesco Vito, 1-00168, Roma, Italy aut Polonelli Luciano Istituto di Microbiologia, Università degli Studi di Parma, Viale Gramsci, 14-43100, Italy aut Antonie van Leeuwenhoek Kluwer Academic Publishers 62/3(1992-10-01), 215-223 0003-6072 62:3<215 1992 62 10482 https://doi.org/10.1007/BF00582582 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00582582 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morace Giulia Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo Francesco Vito, 1-00168, Roma, Italy aut NATIONALLICENCE 700 1- Cattani Paola Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo Francesco Vito, 1-00168, Roma, Italy aut NATIONALLICENCE 700 1- Manzara Stefania Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo Francesco Vito, 1-00168, Roma, Italy aut NATIONALLICENCE 700 1- Conti Stefania Istituto di Microbiologia, Università degli Studi di Parma, Viale Gramsci, 14-43100, Italy aut NATIONALLICENCE 700 1- Gerloni Mara Istituto di Microbiologia, Università degli Studi di Parma, Viale Gramsci, 14-43100, Italy aut NATIONALLICENCE 700 1- Sanguinetti Maurizio Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo Francesco Vito, 1-00168, Roma, Italy aut NATIONALLICENCE 700 1- Posteraro Brunella Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo Francesco Vito, 1-00168, Roma, Italy aut NATIONALLICENCE 700 1- Polonelli Luciano Istituto di Microbiologia, Università degli Studi di Parma, Viale Gramsci, 14-43100, Italy aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 62/3(1992-10-01), 215-223 0003-6072 62:3<215 1992 62 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer