caa a22 4500 469093323 CHVBK 20180323133023.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00572596 doi (NATIONALLICENCE)springer-10.1007/BF00572596 Characterization of a novel enzyme, N6-acetyl-L-lysine: 2-oxoglutarate aminotransferase, which catalyses the second step of lysine catabolism in Candida maltosa [Elektronische Daten] [Heike Schmidt, Rüdiger Bode] A novel aminotransferase catalyzing the second step of lysine catabolism, the oxidative transamination of the α-group of N6-acetyllysine, was identified and characterized in the yeastCandida maltosa. The enzyme was strongly induced in cells grown on L-lysine as sole carbon source. Its activity was specific for both N6-acetyllysine and 2-oxoglutarate. The Km values were 14 mM for the donor, 4 mM for the acceptor and 1.7 μM for pyridoxal-5-phosphate. The enzyme had a maximum activity at pH 8.1 and 32°C. Its molecular mass estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis was 55 kDa. Since the native molecular mass determined by gel filtration was 120 kDa, the enzyme is probably a homodimer. Kluwer Academic Publishers, 1992 Candida maltosa nationallicence N6-acetyl-L-lysine: 2-oxoglutarate aminotransferase nationallicence lysine degradation nationallicence enzyme induction nationallicence Schmidt Heike Institut für Biochemie, Fachrichtung Biologie, Ernst-Moritz-Arndt-Universität Greifswald, 0-2200, Greifswald, Germany aut Bode Rüdiger Institut für Biochemie, Fachrichtung Biologie, Ernst-Moritz-Arndt-Universität Greifswald, 0-2200, Greifswald, Germany aut Antonie van Leeuwenhoek Kluwer Academic Publishers 62/4(1992-11-01), 285-290 0003-6072 62:4<285 1992 62 10482 https://doi.org/10.1007/BF00572596 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00572596 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schmidt Heike Institut für Biochemie, Fachrichtung Biologie, Ernst-Moritz-Arndt-Universität Greifswald, 0-2200, Greifswald, Germany aut NATIONALLICENCE 700 1- Bode Rüdiger Institut für Biochemie, Fachrichtung Biologie, Ernst-Moritz-Arndt-Universität Greifswald, 0-2200, Greifswald, Germany aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 62/4(1992-11-01), 285-290 0003-6072 62:4<285 1992 62 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer