caa a22 4500 469093374 CHVBK 20180323133024.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF00572597 doi (NATIONALLICENCE)springer-10.1007/BF00572597 High-affinity oxygen uptake by Bifidobacterium bifidum [Elektronische Daten] [Raymond Cox, Nils Marling] Oxygen uptake by washed cell suspensions ofBifidobacterium bifidum DSM 20082 was studied by using spectrophotometric measurements of the degree of oxygenation of added myoglobin as a measure of the concentration of dissolved O2. The absorbance changes during consumption of O2 in a closed reaction vessel were analysed by computer to obtain estimates of the changes in dissolved O2 concentration. The cell were then used to calculate the rate of O2 uptake as a function of the dissolved O2 concentration. The cell suspensions showed Michaelis-Menten kinetics with an apparent Km value of 0.06 μM O2. Cell-free extracts contained a soluble NADH oxidase activity with a stoichiometry corresponding to the reduction of O2 to H2O and with a high affinity for O2. Kluwer Academic Publishers, 1992 Bifidobacterium nationallicence NADH oxidase nationallicence oxygen affinity nationallicence oxygen uptake nationallicence Cox Raymond Institute of Biochemistry, Odense University, Campusvej 55, DK 5230, Odense M, Denmark aut Marling Nils Institute of Biochemistry, Odense University, Campusvej 55, DK 5230, Odense M, Denmark aut Antonie van Leeuwenhoek Kluwer Academic Publishers 62/4(1992-11-01), 291-297 0003-6072 62:4<291 1992 62 10482 https://doi.org/10.1007/BF00572597 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00572597 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cox Raymond Institute of Biochemistry, Odense University, Campusvej 55, DK 5230, Odense M, Denmark aut NATIONALLICENCE 700 1- Marling Nils Institute of Biochemistry, Odense University, Campusvej 55, DK 5230, Odense M, Denmark aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 62/4(1992-11-01), 291-297 0003-6072 62:4<291 1992 62 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer