caa a22 4500 469093455 CHVBK 20180323133024.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00584459 doi (NATIONALLICENCE)springer-10.1007/BF00584459 Topoisomerase II: its functions and phosphorylation [Elektronische Daten] [Susan Gasser, Robin Walter, Qi Dang, Maria Cardenas] The gene encoding topoisomerase II in yeast is unique and essential, required for both mitotic and meiotic proliferation. The use of temperature-sensitive mutants in topoisomerase II have demonstrated roles in the relaxation of tortional stress, reduction of recombination rates, and in the separation of sister chromatids after replication. In vertebrate cells, topoisomerase II was shown to be the most abundant component of the metaphase chromosomal scaffold, and has been shown to play a role in chromosome condensationin vitro. The cell cycle control of chromosome condensation may well require phosphorylation of topoisomerase II, since the enzyme is more highly phosphorylated in metaphase than in G1. Recent studies have identified casein kinase II as the major enzyme phosphorylating topoisomerase II in intact yeast cells. The target sites of CKII are exclusively in the C-terminal 400 amino acids of topoisomerase II, the region that is most divergent among the eukaryotic type II enzymes and which is absent in the bacterial gyrase homologues. Kluwer Academic Publishers, 1992 chromosomes nationallicence nuclear scaffold nationallicence phosphorylation nationallicence topoisomerase II nationallicence yeast nationallicence topoII : topoisomerase II nationallicence CKII : Casein Kinase II nationallicence SV40 : Simian Virus 40 nationallicence Gasser Susan Swiss Institute for Experimental Cancer Research (ISREC), CH-1066, Epalinges s/Lausanne, Switzerland aut Walter Robin Swiss Institute for Experimental Cancer Research (ISREC), CH-1066, Epalinges s/Lausanne, Switzerland aut Dang Qi Swiss Institute for Experimental Cancer Research (ISREC), CH-1066, Epalinges s/Lausanne, Switzerland aut Cardenas Maria Swiss Institute for Experimental Cancer Research (ISREC), CH-1066, Epalinges s/Lausanne, Switzerland aut Antonie van Leeuwenhoek Kluwer Academic Publishers 62/1-2(1992-08-01), 15-24 0003-6072 62:1-2<15 1992 62 10482 https://doi.org/10.1007/BF00584459 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00584459 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gasser Susan Swiss Institute for Experimental Cancer Research (ISREC), CH-1066, Epalinges s/Lausanne, Switzerland aut NATIONALLICENCE 700 1- Walter Robin Swiss Institute for Experimental Cancer Research (ISREC), CH-1066, Epalinges s/Lausanne, Switzerland aut NATIONALLICENCE 700 1- Dang Qi Swiss Institute for Experimental Cancer Research (ISREC), CH-1066, Epalinges s/Lausanne, Switzerland aut NATIONALLICENCE 700 1- Cardenas Maria Swiss Institute for Experimental Cancer Research (ISREC), CH-1066, Epalinges s/Lausanne, Switzerland aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 62/1-2(1992-08-01), 15-24 0003-6072 62:1-2<15 1992 62 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer