caa a22 4500 46909348X CHVBK 20180323133024.0 cr unu---uuuuu 170328e19920801xx s 000 0 eng 10.1007/BF00584466 doi (NATIONALLICENCE)springer-10.1007/BF00584466 Thevelein Johan Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit te Leuven, Kardinaal Mercierlaan 92, B-3001, Leuven-Heverlee, Flanders, Belgium aut The RAS-adenylate cyclase pathway and cell cycle control in Saccharomyces cerevisiae [Elektronische Daten] [Johan Thevelein] The cell cycle ofSaccharomyces cerevisiae contains a decision point in G1 called ‘start', which is composed of two specific sites. Nutrient-starved cells arrest at the first site while pheromone-treated cells arrest at the second site. Functioning of the RAS-adenylate cyclase pathway is required for progression over the nutrient-starvation site while overactivation of the pathway renders the cells unable to arrest at this site. However, progression of cycling cells over the nutrient-starvation site does not appear to be triggered by the RAS-adenylate cyclase pathway in response to a specific stimulus, such as an exogenous nutrient. The essential function of the pathway appears to be limited to provision of a basal level of cAMP. cAMP-dependent protein kinase rather than cAMP might be the universal integrator of nutrient availability in yeast. On the other hand stimulation of the pathway in glucose-derepressed yeast cells by rapidly-fermented sugars, such as glucose, is well documented and might play a role in the control of the transition from gluconeogenic growth to fermentative growth. The initial trigger of this signalling pathway is proposed to reside in a ‘glucose sensing complex' which has both a function in controlling the influx of glucose into the cell and in activating in addition to the RAS-adenylate cyclase pathway all other glucose-induced regulatory pathways in yeast. Two crucial problems remaining to be solved with respect to cell cycle control are the nature of the connection between the RAS-adenylate cyclase pathway and nitrogen-source induced progression over the nutrient-starvation site of ‘start' and second the nature of the downstream processes linking the RAS-adenylate cyclase pathway to Cyclin/CDC28 controlled progression over the pheromone site of ‘start'. Kluwer Academic Publishers, 1992 yeast nationallicence cAMP nationallicence growth control nationallicence RAS-oncogene nationallicence general glucose sensor nationallicence signal transduction nationallicence nutrients nationallicence cAMP-PK : cAMP-dependent protein kinase nationallicence Antonie van Leeuwenhoek Kluwer Academic Publishers 62/1-2(1992-08-01), 109-130 0003-6072 62:1-2<109 1992 62 10482 https://doi.org/10.1007/BF00584466 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00584466 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Thevelein Johan Laboratorium voor Moleculaire Celbiologie, Katholieke Universiteit te Leuven, Kardinaal Mercierlaan 92, B-3001, Leuven-Heverlee, Flanders, Belgium aut NATIONALLICENCE 773 0- Antonie van Leeuwenhoek Kluwer Academic Publishers 62/1-2(1992-08-01), 109-130 0003-6072 62:1-2<109 1992 62 10482 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer