caa a22 4500 469093722 CHVBK 20180323133024.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576299 doi (NATIONALLICENCE)springer-10.1007/BF02576299 Influence of colloidal bismuth subcitrate on enzyme secretion from isolated rat pancreatic acinar cells [Elektronische Daten] [T. Kemmer, P. Malfertheiner, G. Bode, H. Ditschuneit] Summary: Bismuth salts are currently used as monotherapy or in combination with antibiotics for the treatment ofHelicobacter pylori-associated peptic ulcer disease. Besides encouraging clinical results with colloidal bismuth subcitrate (CBS), there is an ongoing fear of organ toxicity with the use of bismuth salts. To study potential toxic effects of CBS under short-term exposure, we tested the influence of CBS on amylase secretion from isolated rat pancreatic acinar cells under basal conditions and following carbachol (CCh) and ceruletide (CRT) stimulation. Basal secretion was reduced by 8.9±9.6% (n=10) (mean±SEM) (P<0.05), 5.2±9.2% (P<0.05), 9.4±6.4% (P<0.01), and 6.2±12.2% (P<0.05) with 0.001, 0.01, 0.1, and 1 μg/ml CBS, respectively. With 10 μg/ml and 100 μg/ml CBS, basal amylase secretion was increased in a dose-dependent manner, by 13.7±11.7% (P<0.05) and 24.5±12.8% (P<0.01). CCh (10−5 M)-and CRT (3×10−10 M)-stimulated secretory responses were not altered significantly by any of the CBS doses used. In concentrations above 1 μg/ml, CBS increased pancreatic amylase secretion. Amylase secretion in response to secretagogues was not affected by CBS. These findings are unlikely to be associated with a toxic effect of CBS on exocrine pancreatic acinar cell function. Springer-Verlag, 1992 Bismuth nationallicence Colloidal bismuth subcitrate (CBS) nationallicence Helicobacter pylori nationallicence Exocrine pancreas nationallicence Pancreatic acinar cells nationallicence Kemmer T. Department of Internal Medicine II, University of Ulm, Robert-Koch-Strasse 8, W-7900, Ulm, Federal Republic of Germany aut Malfertheiner P. Department of Internal Medicine II, University of Ulm, Robert-Koch-Strasse 8, W-7900, Ulm, Federal Republic of Germany aut Bode G. Department of Internal Medicine II, University of Ulm, Robert-Koch-Strasse 8, W-7900, Ulm, Federal Republic of Germany aut Ditschuneit H. Department of Internal Medicine II, University of Ulm, Robert-Koch-Strasse 8, W-7900, Ulm, Federal Republic of Germany aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 415-422 0300-9130 192:1<415 1992 192 433 https://doi.org/10.1007/BF02576299 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576299 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kemmer T. Department of Internal Medicine II, University of Ulm, Robert-Koch-Strasse 8, W-7900, Ulm, Federal Republic of Germany aut NATIONALLICENCE 700 1- Malfertheiner P. Department of Internal Medicine II, University of Ulm, Robert-Koch-Strasse 8, W-7900, Ulm, Federal Republic of Germany aut NATIONALLICENCE 700 1- Bode G. Department of Internal Medicine II, University of Ulm, Robert-Koch-Strasse 8, W-7900, Ulm, Federal Republic of Germany aut NATIONALLICENCE 700 1- Ditschuneit H. Department of Internal Medicine II, University of Ulm, Robert-Koch-Strasse 8, W-7900, Ulm, Federal Republic of Germany aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 415-422 0300-9130 192:1<415 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer