caa a22 4500 469093730 CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576265 doi (NATIONALLICENCE)springer-10.1007/BF02576265 Effect of caffeine and zinc on DNA and protein synthesis of neonatal rat cardiac muscle cell in culture [Elektronische Daten] [Y. Kanemaru, M. Rossowska, C. Narayanan, T. Nakamoto] Summary: The effect of caffeine and/or zinc on DNA and protein synthesis of purified neonatal-rat ventricular cardiac myocytes was studied. Caffeine (0.2-2 mM) inhibited both DNA and protein synthesis of the cells. Addition of EDTA in the growth medium inhibited both DNA and protein synthesis. Without caffeine and in the presence of lower concentrations of caffeine (0.2 mM) in the growth medium, 10μM of zinc concentration reversed DNA synthesis, which was inhibited by the chelating agent (EDTA). Higher concentrations of caffeine (2 mM) in the growth medium completely abolished sensitivity of cardiac myocytes to zinc. Additional zinc supplementation to the growth medium of cardiac myocytes did not alter the rate of protein synthesis. The present results suggest that the effect of caffeine on cardiac myocytes may be associated with the zinc-dependent enzymes involved in DNA synthesis. Springer-Verlag, 1992 Myocytes nationallicence Caffeine nationallicence Zinc nationallicence DNA synthesis nationallicence Protein synthesis nationallicence Kanemaru Y. Laboratory of Perinatal Nutrition and Metabolism, Department of Physiology, Louisiana State University Medical Center, 70119, New Orleans, LA, USA aut Rossowska M. Laboratory of Perinatal Nutrition and Metabolism, Department of Physiology, Louisiana State University Medical Center, 70119, New Orleans, LA, USA aut Narayanan C. Department of Anatomy, Louisiana State University Medical Center, 70119, New Orleans, LA, USA aut Nakamoto T. Laboratory of Perinatal Nutrition and Metabolism, Department of Physiology, Louisiana State University Medical Center, 70119, New Orleans, LA, USA aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 115-122 0300-9130 192:1<115 1992 192 433 https://doi.org/10.1007/BF02576265 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576265 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kanemaru Y. Laboratory of Perinatal Nutrition and Metabolism, Department of Physiology, Louisiana State University Medical Center, 70119, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Rossowska M. Laboratory of Perinatal Nutrition and Metabolism, Department of Physiology, Louisiana State University Medical Center, 70119, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Narayanan C. Department of Anatomy, Louisiana State University Medical Center, 70119, New Orleans, LA, USA aut NATIONALLICENCE 700 1- Nakamoto T. Laboratory of Perinatal Nutrition and Metabolism, Department of Physiology, Louisiana State University Medical Center, 70119, New Orleans, LA, USA aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 115-122 0300-9130 192:1<115 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer