caa a22 4500 469093757 CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576261 doi (NATIONALLICENCE)springer-10.1007/BF02576261 Postischemic alteration of muscarinic acetylcholine and adenosine A1 binding sites in gerbil brain [Elektronische Daten] Protective effects of a novel vinca alkaloid derivative, vinconate, and pentobarbital using an autoradiographic study [Tsutomu Araki, Hiroyuki Kato, Kyuya Kogure] Summary: We studied the alterations in the binding of muscarinic cholinergic and adenosine A1 receptors following transient cerebral ischemia in Mongolian gerbils and examined the effects of the novel vinca alkaloid derivative vinconate and pentobarbital against the alterations in the binding of these receptors. Animals were allowed to survive for 5 h and 7 days after 10 min of cerebral ischemia induced by bilateral occlusion of common carotid arteries. [3H]Quinuclidinyl benzilate (QNB) and [3H]cyclohexyladenosine (CHA) were used to label muscarinic cholinergic and adenosine A1 receptors, respectively. The [3H]QNB and [3H]CHA bindings showed no significant alteration in the gerbil brain 5 h after ischemia. However, these bindings in the striatium, the hippocampal CA1 sector, and the hippocampal CA3 sector revealed a significant reduction 7 days after ischemia. The [3H]CHA binding also showed a significant decline in the dentate molecular layer 7 days after ischemia. Intraperitoneal application of vinconate (100 and 300 mg/kg) 10 min and pentobarbital (40 mg/kg) 30 min before ischemia showed a mild reduction in the [3H]CHA binding in the brain 5 h after ischemia. Especially, the reduction was found in the hippocampal CA1 sector and the dentate molecular layer. However, the [3H]QNB binding revealed no significant alteration in the brain 5 h after ischemia. Seven days after ischemia, both drugs prevented a marked reduction in the [3H]CHA binding in the striatium, but not in the hippocampal CA1 sector, the hippocampal CA3 sector, and the dentate molecular layer. By contrast, vinconate and pentobarbital failed to prevent the reduction in the [3H]QNB binding in the striatum. Morphological study indicated that vinconate and pentobarbital ameliorated the neuronal damage to the striatum, but not the hippocampal damage 7 days after ischemia. This histological finding was relatively consistent with the alteration in the [3H]CHA binding. these receptor autoradiographic and histological data suggest that vinconate and pentobarbital can protect the brain from both cellular and functional consequences of ischemia. These findings are of interest in relation to the mechanisms of ischemic brain damage. Springer-Verlag, 1992 Acetylcholine nationallicence Adenosine A1 nationallicence Receptor autoradiography nationallicence Cerebral ischemia nationallicence Vinconate nationallicence Pentobarbital nationallicence Gerbil nationallicence Araki Tsutomu Department of Neurology, Institute of Brain Diseases, Tohoku University School of Medicine, 1-1 Seiryo-machi, Sendai, Japan aut Kato Hiroyuki Department of Neurology, Institute of Brain Diseases, Tohoku University School of Medicine, 1-1 Seiryo-machi, Sendai, Japan aut Kogure Kyuya Department of Neurology, Institute of Brain Diseases, Tohoku University School of Medicine, 1-1 Seiryo-machi, Sendai, Japan aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 79-88 0300-9130 192:1<79 1992 192 433 https://doi.org/10.1007/BF02576261 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576261 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Araki Tsutomu Department of Neurology, Institute of Brain Diseases, Tohoku University School of Medicine, 1-1 Seiryo-machi, Sendai, Japan aut NATIONALLICENCE 700 1- Kato Hiroyuki Department of Neurology, Institute of Brain Diseases, Tohoku University School of Medicine, 1-1 Seiryo-machi, Sendai, Japan aut NATIONALLICENCE 700 1- Kogure Kyuya Department of Neurology, Institute of Brain Diseases, Tohoku University School of Medicine, 1-1 Seiryo-machi, Sendai, Japan aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 79-88 0300-9130 192:1<79 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer