caa a22 4500 469093765 CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576262 doi (NATIONALLICENCE)springer-10.1007/BF02576262 Absence of mitochondrial enhancement in the remnant liver after partial hepatectomy in cirrhotic rats [Elektronische Daten] [T. Morimoto, W. Isselhard] Summary: Changes in energy metabolism after 70% partial hepatectomy were investigated in normal and carbon-tetrachloride-(CCl4)-induced-cirrhotic rats by evaluating hepatic mitochondrial ATP synthesizing activity as well as liver tissue levels of adenine nucleotides and lipid peroxide. Preoperative concentrations of ATP and total adenine nucleotides (TAN: ATP+ADP+AMP) in μmol/g dry weight (dw) and the energy charge potential (ECP) in the cirrhotic livers were 8.53 SEM 0.25, 14.73 SEM 0.54, and 0.74 SEM 0.01, respectively, and were significantly lower than those of normal livers (12.04 SEM 0.34, 15.75 SEM 0.12, and 0.86 SEM 0.01,P<0.01 in TAN). There was no difference in the preoperative mitochondrial phosphorylation rate (PR=×10−10 mol ATP/sec per mg mitochondrial protein) between normal and cirrhotic livers (21.01 SEM 0.95 and 21.55 SEM 1.03, respectively). After hepatectomy, in the normal livers these values decreased slightly after 12 h, remained low until 48 h, and returned to the preoperative value at 72 h. PR was remarkably enhanced and reached the maximum level of 32.54 SEM 2.07 at 24 h (P<0.001, compared to the sham-operated rats) adn gradually returned to the preoperative value at 72 h. In the cirrhotic livers, ATP and ECP levels were drastically decreased at 12 h and recovered to the preoperative levels within 24 h, while TAN level remained unchanged. Enhancement of PR was not observed in any of the cirrhotic livers during the experiment. Lipid peroxidation was transiently increased postoperatively with no difference between normal and cirrhotic livers both in the sham-operated and the hepatectomized rats. These findings suggest that the energy status was more depressed in the cirrhotic livers than in normal livers both before and after hepatectomy. This depressed energy status might be attributed to the low preoperative tissue levels of adenine nucleotides and ECP level in the cirrhotic livers as well as to the absence of the enhancement of PR in the remnant livers. Springer-Verlag, 1992 Liver cirrhosis nationallicence Partial hepatectomy nationallicence Lipid peroxidation nationallicence Energy metabolism nationallicence Mitochondrial function nationallicence Morimoto T. Second Department of Surgery, Faculty of Medicine, Kyoto University, 606, Kyoto, Japan aut Isselhard W. Institute of Experimental Medicine, University of Cologne, Robert-Koch-Strasse 10, W-5000, Köln 41, Federal Republic of Germany aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 89-97 0300-9130 192:1<89 1992 192 433 https://doi.org/10.1007/BF02576262 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576262 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morimoto T. Second Department of Surgery, Faculty of Medicine, Kyoto University, 606, Kyoto, Japan aut NATIONALLICENCE 700 1- Isselhard W. Institute of Experimental Medicine, University of Cologne, Robert-Koch-Strasse 10, W-5000, Köln 41, Federal Republic of Germany aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 89-97 0300-9130 192:1<89 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer