caa a22 4500 46909379X CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576282 doi (NATIONALLICENCE)springer-10.1007/BF02576282 Long term monitoring of immunoreactive endothelin-1 and endothelin-3 in ventricular cerebrospinal fluid, plasma, and 24-h urine of patients with subarachnoid hemorrhage [Elektronische Daten] [Hannelore Ehrenreich, Manfred Lange, Karen Near, Franziska Anneser, Lisa Schoeller, Robert Schmid, Peter Winkler, John Kehrl, Peter Schmiedek, Frank-Detlef Goebel] Summary: Endothelins (ETs), peptides that were originally isolated from endothelial cells, have extremely potent and long-lasting vasoconstricting effects on cerebral vessels in vitro and in vivo. Observations that astrocytes produce these peptides and that their ET production can be stimulated, e.g. by thrombin, and potentiated via a self-enhancing autoregulatory mechanism may have shed new light upon the pathogenesis of cerebrovasospasm (CVS). ETs are present at low levels in normal human cerebrospinal fluid (CSF). Few and contradictory reports exist on ET levels in subarachnoid hemorrhage (SAH)-associated CVS. We monitored ventricular CSF, plasma, and 24-h urine levels of immunoreactive endothelin-1 (ET-1) and endothelin-3 (ET-3) in seven patients with SAH, who did (five) or did not (two) develop CVS in the course of their disease, as well as in two patients with different conditions (acoustic neuroma/postoperative meningitis; hydro-/hematocephalus) over 7-19 days. A distinct peak of both ET-1 and ET-3 in CSF of patients with SAH coincided with clinically documented signs of CVS and was absent in CSF of patients with SAH but no CVS. CSF levels of ET-1 and ET-3 displayed a striking parallelism in all subjects. Plama ET-1 levels were essentially in the normal range. ET-3 was not detectable in plasma under our assay conditions. The excretion profiles of ET-1 and ET-3 in 24-h urine revealed again a predominatly parallel behavior of the two peptides. Interestingly, patients with high ET levels in CSF showed simultaneous peaks in urinary ET excretion, expressed as nanograms per gram of creatinine. Our findings support an association of ETs with the pathogenic events following SAH. The well-documented effects of these peptides on cerebral vessels suggest they are mediators rather than markers of disease. Springer-Verlag, 1992 Endothelin-1 nationallicence Endothelin-3 nationallicence Subarachnoid hemorrhage nationallicence Cerebrovasospasm nationallicence Ehrenreich Hannelore Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA aut Lange Manfred Department of Neurosurgery, Klinikum Grosshadern, Universität München, W-8000, München, Federal Republic of Germany aut Near Karen Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA aut Anneser Franziska Department of Neurosurgery, Klinikum Grosshadern, Universität München, W-8000, München, Federal Republic of Germany aut Schoeller Lisa Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA aut Schmid Robert Department of Neurosurgery, Klinikum Grosshadern, Universität München, W-8000, München, Federal Republic of Germany aut Winkler Peter Department of Neurosurgery, Klinikum Grosshadern, Universität München, W-8000, München, Federal Republic of Germany aut Kehrl John Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA aut Schmiedek Peter Department of Neurosurgery, Klinikum Grosshadern, Universität München, W-8000, München, Federal Republic of Germany aut Goebel Frank-Detlef Medizinische Poliklinik, Universität München, W-8000, München, Federal Republic of Germany aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 257-268 0300-9130 192:1<257 1992 192 433 https://doi.org/10.1007/BF02576282 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576282 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ehrenreich Hannelore Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Lange Manfred Department of Neurosurgery, Klinikum Grosshadern, Universität München, W-8000, München, Federal Republic of Germany aut NATIONALLICENCE 700 1- Near Karen Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Anneser Franziska Department of Neurosurgery, Klinikum Grosshadern, Universität München, W-8000, München, Federal Republic of Germany aut NATIONALLICENCE 700 1- Schoeller Lisa Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Schmid Robert Department of Neurosurgery, Klinikum Grosshadern, Universität München, W-8000, München, Federal Republic of Germany aut NATIONALLICENCE 700 1- Winkler Peter Department of Neurosurgery, Klinikum Grosshadern, Universität München, W-8000, München, Federal Republic of Germany aut NATIONALLICENCE 700 1- Kehrl John Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA aut NATIONALLICENCE 700 1- Schmiedek Peter Department of Neurosurgery, Klinikum Grosshadern, Universität München, W-8000, München, Federal Republic of Germany aut NATIONALLICENCE 700 1- Goebel Frank-Detlef Medizinische Poliklinik, Universität München, W-8000, München, Federal Republic of Germany aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 257-268 0300-9130 192:1<257 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer