caa a22 4500 469093846 CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576260 doi (NATIONALLICENCE)springer-10.1007/BF02576260 Growth inhibition of HIV-1-infected cells and membrane alterations induced by phosphatidylcholine [Elektronische Daten] [A. Willer, K. Buff, A. Kleinschmidt, W. Mellert, F. Goebel, Volker Erfle] Summary: Cultured T-lymphoma cell lines H9 and KE37-1 permanently infected with human immunodeficiency virus (HIV-1, strain HTLV-III B) were exposed to phosphatidylcholine (PC) and a PC-containing formulation "Essentiale” (PC-E). PC and PC-E, but not triglyceride, were found to inhibit growth of virus-infected cells. Additionally, the membrane lipid composition of infected and uninfected H9 cells was investigated upon exposure to PC. The HIV-1-infected cells showed a 25% increase in membrane triglyceride content and a 15% increase in membrane phospholipid saturated fatty acids. In the presence of PC, there is a further increase in triglyceride content up to 180% compared with uninfected control cells, suggsting a possible cause for the selective growth inhibition of HIV-1-infected cells by PC. The PC-E dose range effective in vitro for inhibition of HIV-1-infected cell growth falls within the range that can be reached invivo. Formulations containing PC are well tolerated by humans and might be applicable at an early stage of HIV-1 infection to reduce the number of virus-producing cells. Springer-Verlag, 1992 Human immunodeficiency virus nationallicence HIV-1 nationallicence Growth inhibition nationallicence Phosphatidylcholine nationallicence Willer A. GSF-Abteilung für Molekulare Zellpathologie, Ingolstädter Landstrasse 1, W-8042, Neuherberg/München, Federal Republic of Germany aut Buff K. GSF-Institut für Zellchemie, Ingolstädter Landstrasse 1, W-8042, Neuherberg/München, Federal Republic of Germany aut Kleinschmidt A. GSF-Abteilung für Molekulare Zellpathologie, Ingolstädter Landstrasse 1, W-8042, Neuherberg/München, Federal Republic of Germany aut Mellert W. GSF-Abteilung für Molekulare Zellpathologie, Ingolstädter Landstrasse 1, W-8042, Neuherberg/München, Federal Republic of Germany aut Goebel F. Medizinische Poliklinik der Universität München, Pettenkoferstrasse 8a, W-8000, München 2, Federal Republic of Germany aut Erfle Volker GSF-Abteilung für Molekulare Zellpathologie, Ingolstädter Landstrasse 1, W-8042, Neuherberg/München, Federal Republic of Germany aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 65-77 0300-9130 192:1<65 1992 192 433 https://doi.org/10.1007/BF02576260 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576260 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Willer A. GSF-Abteilung für Molekulare Zellpathologie, Ingolstädter Landstrasse 1, W-8042, Neuherberg/München, Federal Republic of Germany aut NATIONALLICENCE 700 1- Buff K. GSF-Institut für Zellchemie, Ingolstädter Landstrasse 1, W-8042, Neuherberg/München, Federal Republic of Germany aut NATIONALLICENCE 700 1- Kleinschmidt A. GSF-Abteilung für Molekulare Zellpathologie, Ingolstädter Landstrasse 1, W-8042, Neuherberg/München, Federal Republic of Germany aut NATIONALLICENCE 700 1- Mellert W. GSF-Abteilung für Molekulare Zellpathologie, Ingolstädter Landstrasse 1, W-8042, Neuherberg/München, Federal Republic of Germany aut NATIONALLICENCE 700 1- Goebel F. Medizinische Poliklinik der Universität München, Pettenkoferstrasse 8a, W-8000, München 2, Federal Republic of Germany aut NATIONALLICENCE 700 1- Erfle Volker GSF-Abteilung für Molekulare Zellpathologie, Ingolstädter Landstrasse 1, W-8042, Neuherberg/München, Federal Republic of Germany aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 65-77 0300-9130 192:1<65 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer