caa a22 4500 469093854 CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576280 doi (NATIONALLICENCE)springer-10.1007/BF02576280 Relation between the number of sperms to be deposited and pregnancy rate in tubal insemination in rabbits [Elektronische Daten] [K. Kanayama, T. Sankai, T. Endo, Y. Sakuma] Summary: Pregnancy rate of tubal insemination with semen diluted to concentrations of ×100 to ×107 was studied in rabbits. The animals were laparotomized under general anesthesia, and 0.05 ml of diluted semen was injected with a glass capillary from the tubal fimbria into the oviduct. The semen was obtained from mature male rabbits by means of an artificial vagina and diluted to concentrations between ×100 and ×107. One hour after the insemination, ovulation was induced by administration of hCG. Six experimental groups were set up according to the semen concentrations. In the ×100, ×102, ×104, and ×107 groups, newborn rabbits were obtained. The best pregnancy rate was obtained with the ×102 groups, in which 105 level of sperms were deposited. The newborn rabbits were normal not only morphologically, functionally, but also in chromosomal examination. The results suggested that tubal insemination with a small number of sperms could be applied in sterility due to oligospermia in human. Springer-Verlag, 1992 Artificial insemination nationallicence Oviduct nationallicence Rabbit nationallicence Kanayama K. Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Nihon University, 1866 Kameino, 252, Fujisawa, Kanagawa, Japan aut Sankai T. Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Nihon University, 1866 Kameino, 252, Fujisawa, Kanagawa, Japan aut Endo T. Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Nihon University, 1866 Kameino, 252, Fujisawa, Kanagawa, Japan aut Sakuma Y. Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Nihon University, 1866 Kameino, 252, Fujisawa, Kanagawa, Japan aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 241-244 0300-9130 192:1<241 1992 192 433 https://doi.org/10.1007/BF02576280 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576280 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kanayama K. Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Nihon University, 1866 Kameino, 252, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 700 1- Sankai T. Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Nihon University, 1866 Kameino, 252, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 700 1- Endo T. Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Nihon University, 1866 Kameino, 252, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 700 1- Sakuma Y. Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Nihon University, 1866 Kameino, 252, Fujisawa, Kanagawa, Japan aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 241-244 0300-9130 192:1<241 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer