caa a22 4500 469093862 CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576279 doi (NATIONALLICENCE)springer-10.1007/BF02576279 Diurnal variation of amylase secretion is coupled with alterations of β-adrenoceptors in the rat parotid gland [Elektronische Daten] [Y. Ishikawa, I. Amano, C. Chen, H. Ishida] Summary: Diurnal changes in the neurotransmitter receptors are important for studying the receptor function in neurophysiology. The purpose of this study is to gain an insight into the regulatory mechanisms of the diurnal variation of amylase secretion. Rat salivary amylase levels showed a diurnal variation with two peaks, a marked peak at 13 h and a lesser peak at 21 h. This increase in salivary amylase levels was completely inhibited by pretreatment of rats with the β-adrenergic antagonist propranolol, but not by the α-adrenergic antagonist phentolamine. Amylase level in parotid tissue homogenate also showed a diurnal change, but there was only one peak, at 13 h. The number of maximal binding sites (Bmax) for [3H]dihydroalprenolol (DHA) in parotid membranes showed a diurnal variation with two marked peaks at 13 and 21 h, but the affinity of parotid β-adrenoceptors for agonists or antagonists did not show any diurnal changes. Phosphorylation of nuclear non-histone proteins in the rat parotid gland showed diurnal variation with two marked peaks at 13 and 21 h. These results indicate that a diurnal variation in the number of rat parotid β-adrenoceptors, which is presumably regulated by gene expression, is coupled with a change in salivary amylase secretion. Springer-Verlag, 1992 Diurnal variation nationallicence Amylase secretion nationallicence β-Adrenoceptor nationallicence Nonhistone phosphoproteins nationallicence Parotid gland nationallicence Ishikawa Y. Department of Pharmacology, Tokushima University School of Dentistry, 3 Kuramoto-cho, 770, Tokushima-city, Tokushima, Japan aut Amano I. Department of Pharmacology, Tokushima University School of Dentistry, 3 Kuramoto-cho, 770, Tokushima-city, Tokushima, Japan aut Chen C. Department of Pharmacology, Tokushima University School of Dentistry, 3 Kuramoto-cho, 770, Tokushima-city, Tokushima, Japan aut Ishida H. Department of Pharmacology, Tokushima University School of Dentistry, 3 Kuramoto-cho, 770, Tokushima-city, Tokushima, Japan aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 231-240 0300-9130 192:1<231 1992 192 433 https://doi.org/10.1007/BF02576279 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576279 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ishikawa Y. Department of Pharmacology, Tokushima University School of Dentistry, 3 Kuramoto-cho, 770, Tokushima-city, Tokushima, Japan aut NATIONALLICENCE 700 1- Amano I. Department of Pharmacology, Tokushima University School of Dentistry, 3 Kuramoto-cho, 770, Tokushima-city, Tokushima, Japan aut NATIONALLICENCE 700 1- Chen C. Department of Pharmacology, Tokushima University School of Dentistry, 3 Kuramoto-cho, 770, Tokushima-city, Tokushima, Japan aut NATIONALLICENCE 700 1- Ishida H. Department of Pharmacology, Tokushima University School of Dentistry, 3 Kuramoto-cho, 770, Tokushima-city, Tokushima, Japan aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 231-240 0300-9130 192:1<231 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer