caa a22 4500 469093900 CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576281 doi (NATIONALLICENCE)springer-10.1007/BF02576281 Detection of the granulocyte colony-stimulating factor receptor using biotinylated granulocyte colony-stimulating factor: presence of granulocyte colony-stimulating factor receptor on CD34-positive hematopoietic progenitor cells [Elektronische Daten] [K. Shimoda, S. Okamura, N. Harada, Y. Niho] Summary: Granulocyte colony-stimulating factor (G-CSF) was linked to NHS-biotin to yield biotinylated G-CSF (b-G-CSF), which retained the ability to stimulate colony formation by normal bone marrow (BM) cells in methylcellulose. The use of streptivadin-phycoerythrin conjugate in conjunction with flow cytometry demonstrated that the binding of biotnylated G-CSF to its receptor is saturable, competitive, and specific. A 100-fold molar excess of unlabeled G-CSF almost completely inhibited the binding of the biotinylated G-CSF to the human leukemia cell line U937, which is known to posses the G-CSF receptor. G-CSF receptors were clearly detected by flow cytometry on adult human peripheral granulocytes and monocytes, but not on lymphocytes. Using this method, the expression of G-CSF receptors on hematopoietic progenitor cells in bone marrow and umbilical cord blood, detected as CD34-positive (CD34+) cells, were examined. A small but significant number of CD34+ cells were detected among the bone marrow mononuclear cells and umbilical-cord-blood mononuclear cells (4.28%±0.31%, 1.09%±0.20%, respectively). The percentage of CD34+ BM mononuclear cells was significantly higher than for cord blood mononuclear cells (P<0.01). These CD34+ cells were then analyzed by biotinylated G-CSF binding. CD34+ cells from bone marrow contained 25.8%±7.9% G-CSF receptor positive cells and those from cord blood possessed 29.2% ±7.0% of G-CSF receptor-positive cells. The difference was not statistically significant. Springer-Verlag, 1992 G-CSF nationallicence G-CSF receptor nationallicence CD34+ cell nationallicence Flow cytometry nationallicence Shimoda K. The First Department of Internal Medicine, Kyushu University, 812, Fukuoka, Japan aut Okamura S. Cancer Center, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut Harada N. The First Department of Internal Medicine, Kyushu University, 812, Fukuoka, Japan aut Niho Y. The First Department of Internal Medicine, Kyushu University, 812, Fukuoka, Japan aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 245-255 0300-9130 192:1<245 1992 192 433 https://doi.org/10.1007/BF02576281 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576281 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shimoda K. The First Department of Internal Medicine, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Okamura S. Cancer Center, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Harada N. The First Department of Internal Medicine, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Niho Y. The First Department of Internal Medicine, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 245-255 0300-9130 192:1<245 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer