caa a22 4500 469093927 CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576270 doi (NATIONALLICENCE)springer-10.1007/BF02576270 Verapamil attenuates postischemic oxidative injury in the rat liver [Elektronische Daten] [K. Ishii, T. Arima, S. Suita] Summary: We assessed the effects of the calcium channel blocker verapamil on postischemic oxidative injury in the rat liver. In the untreated rats, the values of tissue lipid peroxidation products (thiobarbituric acid-reactive substances) remained unchanged during 90 min of warm ischemia. However, the values increased significantly after the next 60 min of reperfusion compared with those in the sham-operated rats (P<0.01). Intravenous infusion of verapamil (5 μg·kg−1 ·min−1) significantly reduced the extent of lipid peroxidation during reperfusion compared with that in the untreated rats (P<0.02). The percentages of tissue water content and the serum lactate dehydrogenase activities after 60 min of reperfusion were significantly lower in the treated rats than in the untreated rats (P<0.02 andP<0.01, repsectively). We also investigated the influence of verapamil on superoxide-generating activity determined by the superoxide-dependent cytochromec reduction of peritoneal polymorphonuclear leukocytes (PMNs) harvested from normal, non-ischemic, and non-treated rats in vitro. This demonstrated that there was no apparent effect with the highest verapamil concentration level (8μM) observed in the rat plasma during our experiment. These findings suggest that verapamil might reduce the postischemic oxidative injury in the rat liver by mechanisms perhaps not related to the suppression of rat PMNs superoxide-generating activity. Springer-Verlag, 1992 Rat liver nationallicence Postischemic injury nationallicence Polymorphonuclear leukocyte nationallicence Superoxide anion nationallicence Verapamil nationallicence Ishii K. Department of Pediatric Surgery, Faculty of Medicine, Kyushu University Hospital, Maidashi 3-1-1, Higashi-ku, 812, Fukuoka, Japan aut Arima T. Department of Pediatric Surgery, Faculty of Medicine, Kyushu University Hospital, Maidashi 3-1-1, Higashi-ku, 812, Fukuoka, Japan aut Suita S. Department of Pediatric Surgery, Faculty of Medicine, Kyushu University Hospital, Maidashi 3-1-1, Higashi-ku, 812, Fukuoka, Japan aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 151-159 0300-9130 192:1<151 1992 192 433 https://doi.org/10.1007/BF02576270 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576270 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ishii K. Department of Pediatric Surgery, Faculty of Medicine, Kyushu University Hospital, Maidashi 3-1-1, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Arima T. Department of Pediatric Surgery, Faculty of Medicine, Kyushu University Hospital, Maidashi 3-1-1, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Suita S. Department of Pediatric Surgery, Faculty of Medicine, Kyushu University Hospital, Maidashi 3-1-1, Higashi-ku, 812, Fukuoka, Japan aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 151-159 0300-9130 192:1<151 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer