caa a22 4500 46909396X CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576301 doi (NATIONALLICENCE)springer-10.1007/BF02576301 An experimental model for simultaneous quantitative analysis of pulmonary micro- and macrocirculation during unilateral hypoxia in vivo [Elektronische Daten] [Joachim Groh, Gerhard Kuhnle, Wolfgang Kuebler, Alwin Goetz] Summary: An experimental model was developed for quantitative analysis of pulmonary microcirculation using in vivo fluorescence videomicroscopy during unilateral hypoxia induced by one-lung ventilation (1 LV). In five white New Zealand rabbits, pulmonary arterioles on the surface of the right lung were visualized by means of intra-arterial injection of FITC-labeled erythrocytes and FITC-Dextran. During 1 LV of the left lung, the mean airway pressure in the right lung was kept at the level of two-lung ventilation (2 LV) by means of N2-CPAP. Arteriolar diameters as well as parameters of macrocirculation (AP, CVP, PAP, LAP, CO) and gas exchange (paO2, Qs/Qt) were measured simultaneously during 2 LV and 1 LV. FiO2 was kept constant at 1.0 during both experimental phases. Macrohemodynamic parameters during 1 LV did not differ from those measured during 2 LV. 1 LV induced a significant decrease in paO2 (213±105 versus 427±22 mm Hg,P<0.05) and a significant increase in Qs/Qt (22±7 versus 13±2%,P<0.05). During 2 LV (baseline), the pulmonary arteriolar diameters ranged from 15-120 μm. 1 LV resulted in a significant decrease of arteriolar diameters to 89.0±9.3% of baseline (P<0.05). Relative changes in arteriolar diameters were similar for vessels with baseline diameters of 0-40, 40-60, and 60-120 μm (88.4±9.9%, 89.6±9.4%, and 88.4±8.7%, respectively). The present model is the first one allowing in-vivo, investigation of HPV during 1 LV and 2 LV on the basis of simultaneous measurement of pulmonary arteriolar diameters and macrocirculatory parameters in vivo. Although PAP and PVR did not change significantly, a reduction, of pulmonary arteriolar diameters was proven in response to alveolar hypoxia during 1 LV. We suggest the model to be useful in studying the physiological effects of HPV on macro- and microcirculation as well as investigating pathophysiological and pharmacological influences on HPV. Springer-Verlag, 1992 Pulmonary circulation nationallicence Intravital fluorescence microscopy nationallicence Hypoxic pulmonary vasoconstriction nationallicence One-lung ventilation nationallicence Rabbit nationallicence Groh Joachim Institut für Anaesthesiologie, Ludwig-Maximilians-Universität, Klinikum Großhadern, Marchioninistrasse 15, W-8000, München 70, Federal Republic of Germany aut Kuhnle Gerhard Institut für Chirurgische Forschung, Ludwig-Maximilians-Universität, Klinikum Großhadern, Marchioninistrasse 15, W-8000, München 70, Federal Republic of Germany aut Kuebler Wolfgang Institut für Chirurgische Forschung, Ludwig-Maximilians-Universität, Klinikum Großhadern, Marchioninistrasse 15, W-8000, München 70, Federal Republic of Germany aut Goetz Alwin Institut für Anaesthesiologie, Ludwig-Maximilians-Universität, Klinikum Großhadern, Marchioninistrasse 15, W-8000, München 70, Federal Republic of Germany aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 431-441 0300-9130 192:1<431 1992 192 433 https://doi.org/10.1007/BF02576301 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576301 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Groh Joachim Institut für Anaesthesiologie, Ludwig-Maximilians-Universität, Klinikum Großhadern, Marchioninistrasse 15, W-8000, München 70, Federal Republic of Germany aut NATIONALLICENCE 700 1- Kuhnle Gerhard Institut für Chirurgische Forschung, Ludwig-Maximilians-Universität, Klinikum Großhadern, Marchioninistrasse 15, W-8000, München 70, Federal Republic of Germany aut NATIONALLICENCE 700 1- Kuebler Wolfgang Institut für Chirurgische Forschung, Ludwig-Maximilians-Universität, Klinikum Großhadern, Marchioninistrasse 15, W-8000, München 70, Federal Republic of Germany aut NATIONALLICENCE 700 1- Goetz Alwin Institut für Anaesthesiologie, Ludwig-Maximilians-Universität, Klinikum Großhadern, Marchioninistrasse 15, W-8000, München 70, Federal Republic of Germany aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 431-441 0300-9130 192:1<431 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer