caa a22 4500 469093978 CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576269 doi (NATIONALLICENCE)springer-10.1007/BF02576269 Regulation of NK cell activity and the level of the intracellular cAMP in human peripheral blood lymphocytes by Met-enkephalin [Elektronische Daten] [I. Martin-Kleiner, M. Osmak, J. Gabrilovac] Summary: The effect of Met-enkephalin on natural killer (NK) activity and on intracellular cyclic adenosine monophosphate (cAMP) level in human peripheral blood lymphocytes was measured 1/4, 1, 2, and 24h after incubation. Concentrations of Met-enkephalin ranged from 10−12 to 10−8 M. Cyclic changes in NK activity and in the intracellular cAMP level after treatment with Met-enkephalin were observed. Kinetics of changes caused by high (10−9, 10−8 M) and low concentrations (10−12, 10−11, 10−10 M) of Met-enkephalin differed from each other. Early, nearly threefold increase in the level of intracellular cAMP was found 15 min after treating peripheral blood lymphocytes with 10−12 M Met-enkephalin. By contrast, a nearly 75% decrease of intracellular cAMP level was found 2 h after treatment with 10−9 M Met-enkephalin. Generally, early intensive changes in the cAMP level in peripheral blood lymphocytes, induced by Met-enkephalin, were followed by delayed, subtle changes in NK cell activity in the opposite direction. Springer-Verlag, 1992 Met-enkephalin nationallicence NK cell activity nationallicence cAMP nationallicence Peripheral blood lymphocytes nationallicence Martin-Kleiner I. Department of Experimental Biology and Medicine, Ruder Bošković Institute, Bijenička 54, P.O. Box 1016, 41001, Zagreb, Croatia aut Osmak M. Department of Experimental Biology and Medicine, Ruder Bošković Institute, Bijenička 54, P.O. Box 1016, 41001, Zagreb, Croatia aut Gabrilovac J. Department of Experimental Biology and Medicine, Ruder Bošković Institute, Bijenička 54, P.O. Box 1016, 41001, Zagreb, Croatia aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 145-150 0300-9130 192:1<145 1992 192 433 https://doi.org/10.1007/BF02576269 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576269 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Martin-Kleiner I. Department of Experimental Biology and Medicine, Ruder Bošković Institute, Bijenička 54, P.O. Box 1016, 41001, Zagreb, Croatia aut NATIONALLICENCE 700 1- Osmak M. Department of Experimental Biology and Medicine, Ruder Bošković Institute, Bijenička 54, P.O. Box 1016, 41001, Zagreb, Croatia aut NATIONALLICENCE 700 1- Gabrilovac J. Department of Experimental Biology and Medicine, Ruder Bošković Institute, Bijenička 54, P.O. Box 1016, 41001, Zagreb, Croatia aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 145-150 0300-9130 192:1<145 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer