caa a22 4500 469093986 CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576275 doi (NATIONALLICENCE)springer-10.1007/BF02576275 Reduced clearance of leukocytes from lungs in septic rats [Elektronische Daten] [L. Hansson, J. Thörne, B. Jeppsson] Summary: Leukocytes have previously been shown to sequestrate in the lungs and liver in association with traumatic and septic shock. In a rat model of gram-negative sepsis of intra-abdominal origin, a previously described in vivo technique was used for dynamic studies of leukocyte sequestration in different organs using white blood cells labeled with 111-indium-oxine. One group of rats was either studied immediately after induction of sepsis or for 6 h under a scintillation camera for continuous registration of the activity distribution (i.e., presence of leukocytes). Another group was studied 12 h after induction of sepsis for 60 min. The activity increased immediately over the lungs, indicating sequestration of the leukocytes during the first 6 h, but there was no significant difference in this respect between septic and control animals. It does not seem possible to study leukocyte sequestration dynamically in this way. When the labeled leukocytes were administered 12 h after induction of sepsis, however, the activity of septic animals' lungs was seen to remain elevated over the time period studied compared with control rats, in which the activity slowly decreased. In the liver and spleen, the activity increased in both groups, but significantly more so in control animals, which may be explained by disturbed leukocyte margination and cell turnover in the septic animals. This study has indicated that leukocyte distribution in different organs is affected by sepsis and this reaction can be studied using radiolabeled leukocytes. Springer-Verlag, 1992 Experimental sepsis nationallicence ARDS nationallicence Labeled leukocytes nationallicence Hansson L. Department of Surgery, Lund University, S-22185, Lund, Sweden aut Thörne J. Department of Surgery, Lund University, S-22185, Lund, Sweden aut Jeppsson B. Department of Surgery, Lund University, S-22185, Lund, Sweden aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 197-204 0300-9130 192:1<197 1992 192 433 https://doi.org/10.1007/BF02576275 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576275 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hansson L. Department of Surgery, Lund University, S-22185, Lund, Sweden aut NATIONALLICENCE 700 1- Thörne J. Department of Surgery, Lund University, S-22185, Lund, Sweden aut NATIONALLICENCE 700 1- Jeppsson B. Department of Surgery, Lund University, S-22185, Lund, Sweden aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 197-204 0300-9130 192:1<197 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer