caa a22 4500 469094001 CHVBK 20180323133025.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576291 doi (NATIONALLICENCE)springer-10.1007/BF02576291 Simulated weightlessness and bone metabolism: gravitational stimulation enhances insulin sensitivity [Elektronische Daten] [M. Yamaguchi, T. Hoshi] Summary: The effect of simulated weightlessness on bone metabolism was investigated in skeletal unloading for 4 days. Skeletal unloading was designed using the model of hindlimb hang in rats. Skeletal unloading with hindlimb hang cased a significant decrease of alkaline phosphatase activity, deoxyribonucleic acid (DNA) content, and glucose consumption in the femoral diaphysis, but not in the calvaria. When femoral-diaphyseal tissues were cultured in the presence of insulin (10−8 M), the hormone produced a significant increase of alkaline phosphatase activity and decrease of glucose consumption in the femoral-diaphyseal tissues obtained from normal rats. This hormonal effect was not seen in the femoral diaphysis, but in the calvaria, of rats with skeletal unloading. However, insulin effect was seen in the femoral diaphysis obtained at 3 days after the removal of skeletal unloading. Meanwhile, the presence of other bone-regulating factors (10−8 M parathyroid hormone [1-34] and 10−4 M zinc sulfate) revealed an appreaciable effect on alkaline phosphatase activity in the femoral diaphysis from rats with skeletal unloading. These results suggest that gravitational stimulation can directly enhance a specific insulin sensitivity in the regulation of bone metabolism. Springer-Verlag, 1992 Weightlessness nationallicence Skeletal unloading nationallicence Bone metabolism nationallicence Insulin nationallicence Rat femur nationallicence Yamaguchi M. Laboratory of Metabolism and Endocrinology, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut Hoshi T. Laboratory of Physiology, School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 345-353 0300-9130 192:1<345 1992 192 433 https://doi.org/10.1007/BF02576291 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576291 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaguchi M. Laboratory of Metabolism and Endocrinology, Graduate School of Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut NATIONALLICENCE 700 1- Hoshi T. Laboratory of Physiology, School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, 422, Shizuoka City, Japan aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 345-353 0300-9130 192:1<345 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer