caa a22 4500 469094036 CHVBK 20180323133026.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576274 doi (NATIONALLICENCE)springer-10.1007/BF02576274 Photosensitization of human bladder carcinoma cells by pyrene-dodecanoic acid: quantitative analysis of the cytotoxicity [Elektronische Daten] [Eitan Fibach, Eliezer Rachmilewitz, Shimon Gatt] Summary: The effects of photochemotherapy with the fluroescent fatty acid pyrenedodecanoic acid (P12) and long-wavelength ultraviolet (UVA) light on cells derived from human bladder carcinoma were studied. Exposure of these anchorage-dependent cells to P12 either in monolayers of adherent cells or in suspension resulted in a time-related uptake of P12 and its incorporation into the cells' neutral and phospholipids. The uptake and localization of P12 was visualized with fluorescence microscopy and the distribution of the cell population with respect to P12 uptake was analyzed by flow cytometry. Irradiation of P12-containing monolayers of bladder carcinoma cells with UVA light resulted in cell killing. But, on microscopic examination no apparent cell lysis was detected, and since digestion with trypsin did not result in the dispersion of the monolayers it was impossible to assess toxicity by cell count. Alternative procedures were therefore used, and the following cell parameters were determined: (a) cellular uptake or release of chromate; (b) ability of cells to re-adhere to the substratum; and (c) the long-range proliferation potential. The combined inhibitory effect of photoirradiation on cell adherence and on their proliferative potential was utilized for determining reductions of up to 7 log in cell viability. The results obtained with five independently established in vitro bladder carcinoma cell lines indicated that these cells are susceptible to P12-induced photosensitization, suggesting that bladder malignancies might be potential candidates for pyrene-induced photochemotherapy. Springer-Verlag, 1992 Carcinoma in situ nationallicence Photosensitization nationallicence Photodynamic therapy nationallicence Fatty acids nationallicence Fibach Eitan Department of Hematology, Hadassah University Hospital, 91120, Jerusalem, Israel aut Rachmilewitz Eliezer Department of Hematology, Hadassah University Hospital, 91120, Jerusalem, Israel aut Gatt Shimon Department of Membrane Biochemistry and Neurochemistry, Hebrew University-Hadassah School of Medicine, POB 12000, 91120, Jerusalem, Israel aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 185-196 0300-9130 192:1<185 1992 192 433 https://doi.org/10.1007/BF02576274 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576274 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fibach Eitan Department of Hematology, Hadassah University Hospital, 91120, Jerusalem, Israel aut NATIONALLICENCE 700 1- Rachmilewitz Eliezer Department of Hematology, Hadassah University Hospital, 91120, Jerusalem, Israel aut NATIONALLICENCE 700 1- Gatt Shimon Department of Membrane Biochemistry and Neurochemistry, Hebrew University-Hadassah School of Medicine, POB 12000, 91120, Jerusalem, Israel aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 185-196 0300-9130 192:1<185 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer