caa a22 4500 469094087 CHVBK 20180323133026.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576259 doi (NATIONALLICENCE)springer-10.1007/BF02576259 Influence of ethanol on survival of acinar cells isolated from rat pancreas [Elektronische Daten] [M. Siech, G. Letko] Summary: The role of ethanol in precipitating acute pancreatitis has been studied in model experiments. The influence of ethanol on the survival of isolated pancreatic acinar cells (PAC) and a possible coaction with noxious agents (such as trypsin, chymotrypsin, temporary anoxia, and partial uncoupling of the oxidative phosphorylation by 2,4-dinitrophenol [DNP]) has been investigated. Isolated PACs withstood ethanol levels up to 180 mM without significant decrease of viability within 4 h incubation at 37°C. A 90-min deprivation of oxygen was widely tolerated also. However, with a combination of both ethanol application and oxygen deprivation, a clearly forced cell damage was observed as was true with a combination of ethanol and chymotrypsin. The action of DNP or of extracellular trypsin on cell survival was not amplified by the addition of ethanol (180 mM). This study reveals two possible sites of action at which ethanol may contribute to the pathogenesis of acute pancreatitis. Springer-Verlag, 1992 Acinar cell nationallicence Acute pancreatitis nationallicence Ethanol nationallicence Anoxia nationallicence Trypsin nationallicence Chymotrypsin nationallicence Siech M. Division of Experimental Surgery of the Clinic of Surgery, Medical Academy of Magdeburg, O-3090, Magdeburg, Federal Republic of Germany aut Letko G. Division of Experimental Surgery of the Clinic of Surgery, Medical Academy of Magdeburg, O-3090, Magdeburg, Federal Republic of Germany aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 57-63 0300-9130 192:1<57 1992 192 433 https://doi.org/10.1007/BF02576259 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576259 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Siech M. Division of Experimental Surgery of the Clinic of Surgery, Medical Academy of Magdeburg, O-3090, Magdeburg, Federal Republic of Germany aut NATIONALLICENCE 700 1- Letko G. Division of Experimental Surgery of the Clinic of Surgery, Medical Academy of Magdeburg, O-3090, Magdeburg, Federal Republic of Germany aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 57-63 0300-9130 192:1<57 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer