caa a22 4500 469094109 CHVBK 20180323133026.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576286 doi (NATIONALLICENCE)springer-10.1007/BF02576286 Phorbol ester attenuates inositol 1,4,5-trisphosphate-induced Ca2+ release in electropermeabilized rat pancreatic acini [Elektronische Daten] [Y. Arita, T. Kimura, Y. Ogami, H. Nawata] Summary: To investigate the mechanism of inositol trisphosphate (IP3)-induced Ca2+ release from the internal Ca2+ store, we examined the effects of heparin, phorbol ester and cyclic nucleotides on Ca2+ release induced by carbachol or inositol 1,4,5-trisphosphate (1,4,5-IP3). For monitoring changes of Ca2+ we used the fluorescent indicator, fura-2, in electropermeabilized rat pancreatic acini. An amount of 100 μg/ml heparin inhibited the Ca2+ release induced by 1 μM 1,4,5-IP3 in permeabilized acini. Pretreatment with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) for 10 min reduced the release of Ca2+ induced by 10 μM carbachol and 1 μM 1,4,5-IP3 in permeabilized acini. Staurosporine, a protein kinase C inhibitor, blocked the inhibitory effect of TPA. Cytosolic calcium concentration was restored by staurosporine in TPA-treated acini. Although cyclic AMP exaggerated the amylase release induced by carbachol, cyclic AMP and cyclic GMP had no effect on the carbachol-induced release of Ca2+ in permeabilized acini. These findings suggest that protein kinase C may act at the level of the IP3 receptors or the IP3-operated Ca2+ channels of the internal Ca2+ store and indicate that cyclic nucleotides do not affect the IP3-induced release of Ca2+ in rat pancreatic acini. Springer-Verlag, 1992 Exocrine pancreas nationallicence Phorbol ester nationallicence Calcium mobilization nationallicence Cyclic nucleotide nationallicence Permeabilized cell nationallicence Arita Y. The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut Kimura T. The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut Ogami Y. The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut Nawata H. The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 295-303 0300-9130 192:1<295 1992 192 433 https://doi.org/10.1007/BF02576286 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576286 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arita Y. The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Kimura T. The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Ogami Y. The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 700 1- Nawata H. The Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, 812, Fukuoka, Japan aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 295-303 0300-9130 192:1<295 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer