caa a22 4500 469094192 CHVBK 20180323133026.0 cr unu---uuuuu 170328e19921201xx s 000 0 eng 10.1007/BF02576300 doi (NATIONALLICENCE)springer-10.1007/BF02576300 Effects of lipoxygenase and cycloxygenase inhibitors on murine antibody-dependent cellular cytotoxicity (ADCC) [Elektronische Daten] [J. Hernández-Godoy, D. Planelles, A. Bayona, B. Balsalobre, A. González-Molina] Summary: The purpose of this study was to determine the effects that specific inhibition of arachidonic acid (AA) metabolism has on the antibody-dependent cellular cytotoxicity (ADCC) of murine spleen cells. The action of three inhibitors of the lipoxygenase (LO) pathway—nordihydroguaiaretic acid (NDGA), esculetin (Es), and phenantroline (Phe)—was compared with that of three inhibitors of the cycloxygenase (CO) pathway—indomethacin (INDO), acetyl salicylic acid (ASA), and imidazole (IMI). All the LO inhibitors suppressed ADCC function in a dose-dependent manner, but NDGA was the most potent inhibitor of this cytolytic activity. In fact, NDGA inhibited the ADCC function with 97% inhibition at 10μM, while Phe and Es, at the same concentration, inhibited ADCC by 21% and 19%, respectively. However, CO inhibitors did not markedly affect ADCC function and only some doses of them had a slight, but significant, depressing effect (8-11% inhibition at 0.01-0.1 μM of INDO, 7% inhibition at 400μM of ASA, and 13% inhibition at 800-1000μM of IMI). These results suggest the LO pathway of the arachidonic acid metabolism plays an important role in regulating ADCC activity of murine spleen cells and the products of the CO pathway have little effect on ADCC lysis. Springer-Verlag, 1992 ADCC nationallicence Arachidonic acid nationallicence Lipoxygenase nationallicence Cycloxygenase nationallicence Mice nationallicence Hernández-Godoy J. Unidad de Inmunologia Experimental, Centro de Investigación, Hospital La Fe, Avenida Campanar No. 21, E-46009, Valencia, Spain aut Planelles D. Unidad de Inmunologia Experimental, Centro de Investigación, Hospital La Fe, Avenida Campanar No. 21, E-46009, Valencia, Spain aut Bayona A. Unidad de Inmunologia Experimental, Centro de Investigación, Hospital La Fe, Avenida Campanar No. 21, E-46009, Valencia, Spain aut Balsalobre B. Unidad de Inmunologia Experimental, Centro de Investigación, Hospital La Fe, Avenida Campanar No. 21, E-46009, Valencia, Spain aut González-Molina A. Unidad de Inmunologia Experimental, Centro de Investigación, Hospital La Fe, Avenida Campanar No. 21, E-46009, Valencia, Spain aut Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 423-430 0300-9130 192:1<423 1992 192 433 https://doi.org/10.1007/BF02576300 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02576300 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hernández-Godoy J. Unidad de Inmunologia Experimental, Centro de Investigación, Hospital La Fe, Avenida Campanar No. 21, E-46009, Valencia, Spain aut NATIONALLICENCE 700 1- Planelles D. Unidad de Inmunologia Experimental, Centro de Investigación, Hospital La Fe, Avenida Campanar No. 21, E-46009, Valencia, Spain aut NATIONALLICENCE 700 1- Bayona A. Unidad de Inmunologia Experimental, Centro de Investigación, Hospital La Fe, Avenida Campanar No. 21, E-46009, Valencia, Spain aut NATIONALLICENCE 700 1- Balsalobre B. Unidad de Inmunologia Experimental, Centro de Investigación, Hospital La Fe, Avenida Campanar No. 21, E-46009, Valencia, Spain aut NATIONALLICENCE 700 1- González-Molina A. Unidad de Inmunologia Experimental, Centro de Investigación, Hospital La Fe, Avenida Campanar No. 21, E-46009, Valencia, Spain aut NATIONALLICENCE 773 0- Research in Experimental Medicine Springer-Verlag 192/1(1992-12-01), 423-430 0300-9130 192:1<423 1992 192 433 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer