caa a22 4500 46910192X CHVBK 20180323133046.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00189633 doi (NATIONALLICENCE)springer-10.1007/BF00189633 A mechanistic perspective on bacterial metabolism of chlorinated methanes [Elektronische Daten] [Lawrence Wackett, Michael Logan, Frank Blocki, Cai Bao-li] Chlorinated methanes are important environmental pollutants, which can be metabolized by bacteria. The biotransformation of chlorinated methanes by bacteria has been shown to be due either to gratuitous metabolism (cometabolism) or their use as a source of carbon and energy. The reactions which result in carbon-halogen bond cleavage include substitutive, reductive, oxygenative, and gem-elimination mechanisms. Certain methylotrophic bacteria can use dichloromethane as a source of carbon and energy. Dichloromethane dehalogenase catalyzes the first substitutive reaction in this metabolism. The enzyme shows a 1010-fold rate enhancement over the reaction of the bisulfide anion with dichloromethane in water. Pseudomonas putida G786 synthesizes cytochrome P-450CAM which catalyzes the gratuitous reduction of chlorinated methanes. These studies with purified enzymes are beginning to reveal more detailed mechanistic features of bacterial chlorinated methane metabolism. Kluwer Academic Publishers, 1992 chloromethanes nationallicence chlorofluoromethanes nationallicence mechanisms nationallicence bacteria nationallicence dehalogenation nationallicence biodegradation nationallicence DNA : deoxyribonucleic acid nationallicence kcat : catalytic first order rate constant for an enzyme catalyzed reaction nationallicence KM : Michaelis constant for an enzyme catalyzed reaction nationallicence MNDO : modified neglect of diatomic overlap nationallicence PIMA : pattern induced multialignment nationallicence DCMD : dichloromethane dehalogenase nationallicence Wackett Lawrence Department of Biochemistry, University of Minnesota, 55108, St. Paul, MN, USA aut Logan Michael Department of Biochemistry, University of Minnesota, 55108, St. Paul, MN, USA aut Blocki Frank Department of Biochemistry, University of Minnesota, 55108, St. Paul, MN, USA aut Bao-li Cai Institute of Molecular Biology, Nankai University, Tianjin, People's Republic of China aut Biodegradation Kluwer Academic Publishers 3/1(1992-03-01), 19-36 0923-9820 3:1<19 1992 3 10532 https://doi.org/10.1007/BF00189633 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00189633 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wackett Lawrence Department of Biochemistry, University of Minnesota, 55108, St. Paul, MN, USA aut NATIONALLICENCE 700 1- Logan Michael Department of Biochemistry, University of Minnesota, 55108, St. Paul, MN, USA aut NATIONALLICENCE 700 1- Blocki Frank Department of Biochemistry, University of Minnesota, 55108, St. Paul, MN, USA aut NATIONALLICENCE 700 1- Bao-li Cai Institute of Molecular Biology, Nankai University, Tianjin, People's Republic of China aut NATIONALLICENCE 773 0- Biodegradation Kluwer Academic Publishers 3/1(1992-03-01), 19-36 0923-9820 3:1<19 1992 3 10532 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer