caa a22 4500 469101938 CHVBK 20180323133046.0 cr unu---uuuuu 170328e19920301xx s 000 0 eng 10.1007/BF00189639 doi (NATIONALLICENCE)springer-10.1007/BF00189639 Anaerobic degradation of tetrachloromethane by Acetobacterium woodii [Elektronische Daten] separation of dechlorinative activities in cell extracts and roles for vitamin B12 and other factors [Susanna Stromeyer, Karin Stumpf, Alasdair Cook, Thomas Leisinger] The transformations of 14CCl4 by whole cells of Acetobacterium woodii suspended in phosphate buffer containing reducing agents, and by the cobalt corrinoid aquocobalamin in the same solution, were compared. Each catalyst transformed 14CCl4 not only to reduced products (CHCl3 and CH2Cl2) but also to CO and CO2 as well as non-volatile products. The mass balance for radioactive carbon was complete in each case. Thus, the reactions of the pure cobalt corrinoid resemble the reactions in vivo. The proton in CHCl3, formed from CCl4 by A. woodii, was derived from water. Extracts of A. woodii were fractionated into large and small molecules, and each of the two fractions was separated chromatographically. Fractions of proteins demonstrated poor correlation between content of the corrinoid vitamin B12 and rates of transformation of CCl4. The correlation was somewhat improved if the fractions were autoclaved, but dechlorination in the absence of vitamin B12 was observed. Separation of the small molecules yielded only one fraction containing vitamin B12, and this fraction catalyzed dechlorination, whereas several other fractions were able to dechlorinate CCl4 in the absence of vitamin B12. We presume there to be unrecognized dechlorinative factors in anaerobic bacteria. Kluwer Academic Publishers, 1992 tetrachloromethane nationallicence dechlorination by non-enzymic catalysts nationallicence chlorinated aliphatic solvents nationallicence anaerobic biotransformation nationallicence dechlorination by reduced corrinoids nationallicence GC : gas chromatograph(y) nationallicence GC-MS (or-TCD or-FID) : GC coupled to a mass spectrometer (or a thermal conductivity detector or a flame ionization detector) nationallicence HPLC : high pressure liquid chromatograph(y) nationallicence FPLC : high pressure protein chromatograph(y) nationallicence Stromeyer Susanna Microbiology Institute, Swiss Federal Institute of Technology, CH-8092, Zürich, Switzerland aut Stumpf Karin Microbiology Institute, Swiss Federal Institute of Technology, CH-8092, Zürich, Switzerland aut Cook Alasdair Microbiology Institute, Swiss Federal Institute of Technology, CH-8092, Zürich, Switzerland aut Leisinger Thomas Microbiology Institute, Swiss Federal Institute of Technology, CH-8092, Zürich, Switzerland aut Biodegradation Kluwer Academic Publishers 3/1(1992-03-01), 113-123 0923-9820 3:1<113 1992 3 10532 https://doi.org/10.1007/BF00189639 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00189639 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stromeyer Susanna Microbiology Institute, Swiss Federal Institute of Technology, CH-8092, Zürich, Switzerland aut NATIONALLICENCE 700 1- Stumpf Karin Microbiology Institute, Swiss Federal Institute of Technology, CH-8092, Zürich, Switzerland aut NATIONALLICENCE 700 1- Cook Alasdair Microbiology Institute, Swiss Federal Institute of Technology, CH-8092, Zürich, Switzerland aut NATIONALLICENCE 700 1- Leisinger Thomas Microbiology Institute, Swiss Federal Institute of Technology, CH-8092, Zürich, Switzerland aut NATIONALLICENCE 773 0- Biodegradation Kluwer Academic Publishers 3/1(1992-03-01), 113-123 0923-9820 3:1<113 1992 3 10532 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer