caa a22 4500 469102063 CHVBK 20180323133047.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00129089 doi (NATIONALLICENCE)springer-10.1007/BF00129089 Bacterial PCB biodegradation [Elektronische Daten] [Alfred Boyle, Christopher Silvin, John Hassett, James Nakas, S. Tanenbaum] The environment has become polluted with a variety of xenobiotics, including PCBs, as a result of the industrial development of useful halogenated compounds. While the PCBs may not exhibit the acute toxicity originally ascribed to them, they and their attendant byproducts remain as significant factors for adverse effects in the ecological food-chain. The use of microorganisms for bioremediation of PCBs is reviewed. This paper further details three new isolates obtained by conventional enrichment technics which show significant degradation capabilities for Aroclor 1242. These were identified by morphology, staining, and fatty acid analysis as Comamonas testosteroni, Rhodococcus rhodochrous, and a strain of Pseudomonas putida. These isolates demonstrated somewhat selective degradations of the congeners within Aroclor 1242; comprising total losses of 13.8, 19.1, and 24.6%, respectively. Each organism can attack dichloro-through tetrachlorobiphenyls. Analysis of chromatographic patterns from anaerobically digested Aroclor 1242 samples treated by these bacteria demonstrated decreases in di- through penta-substituted biphenyls. Each of these isolates, with discrete specificities, showed preferences for ‘open' 2,3-sites, indicative of the action of 2,3-dioxygenase enzymes. The identification of many intermediates in the foregoing transformations was established by GC-MS analyses. Several variations in metabolic pathways, centering on the meta cleavage product 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) were suggested from these data. It is concluded that the described strains may be of future bioremediation use in processes which have an initial anaerobic dechlorination stage. Kluwer Academic Publishers, 1992 PCB biodegradation nationallicence bacterial metabolism nationallicence Comamonas nationallicence Pseudomonas nationallicence Rhodococcus spp nationallicence anaerobic/anaerobic degradative pathways nationallicence environmental considerations nationallicence Boyle Alfred College of Environmental Science & Forestry, State University of New York, 13210, Syracuse, NY, USA aut Silvin Christopher College of Environmental Science & Forestry, State University of New York, 13210, Syracuse, NY, USA aut Hassett John College of Environmental Science & Forestry, State University of New York, 13210, Syracuse, NY, USA aut Nakas James College of Environmental Science & Forestry, State University of New York, 13210, Syracuse, NY, USA aut Tanenbaum S. College of Environmental Science & Forestry, State University of New York, 13210, Syracuse, NY, USA aut Biodegradation Kluwer Academic Publishers 3/2-3(1992-06-01), 285-298 0923-9820 3:2-3<285 1992 3 10532 https://doi.org/10.1007/BF00129089 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00129089 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Boyle Alfred College of Environmental Science & Forestry, State University of New York, 13210, Syracuse, NY, USA aut NATIONALLICENCE 700 1- Silvin Christopher College of Environmental Science & Forestry, State University of New York, 13210, Syracuse, NY, USA aut NATIONALLICENCE 700 1- Hassett John College of Environmental Science & Forestry, State University of New York, 13210, Syracuse, NY, USA aut NATIONALLICENCE 700 1- Nakas James College of Environmental Science & Forestry, State University of New York, 13210, Syracuse, NY, USA aut NATIONALLICENCE 700 1- Tanenbaum S. College of Environmental Science & Forestry, State University of New York, 13210, Syracuse, NY, USA aut NATIONALLICENCE 773 0- Biodegradation Kluwer Academic Publishers 3/2-3(1992-06-01), 285-298 0923-9820 3:2-3<285 1992 3 10532 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer