caa a22 4500 469102071 CHVBK 20180323133047.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1007/BF00129079 doi (NATIONALLICENCE)springer-10.1007/BF00129079 Atlas Ronald Department of Biology, University of Louisville, 40292, Louisville, KY, USA aut Molecular methods for environmental monitoring and containment of genetically engineered microorganisms [Elektronische Daten] [Ronald Atlas] Plans to introduce genetically engineered microorganisms into the environment has led to concerns over safety and has raised questions about how to detect and to contain such microorganisms. Specific gene sequences, such as lacZ, have been inserted into genetically engineered microorganisms to permit their phenotypic detection. Molecular methods have been developed based upon recovery of DNA from environmental samples and gene probe hybridization to specific diagnostic gene sequences for the specific detection of genetically engineered microorganisms. DNA amplification using the polymerase chain reaction has been applied to enhance detection sensitivity so that single gene targets can be detected. Detection of messenger RNA has permitted the monitoring of gene expression in the environment. The use of reporter genes, such as the lux gene for bioluminescence, likewise has permitted the observation of gene expression. Conditional lethal constructs have been developed as models for containment of genetically engineered microorganisms. Suicide vectors, based upon the hok gene have been developed as model containment systems. Kluwer Academic Publishers, 1992 genetically engineered microorganisms nationallicence gene probes nationallicence polymerase chain reaction nationallicence suicide vectors nationallicence Biodegradation Kluwer Academic Publishers 3/2-3(1992-06-01), 137-146 0923-9820 3:2-3<137 1992 3 10532 https://doi.org/10.1007/BF00129079 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00129079 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Atlas Ronald Department of Biology, University of Louisville, 40292, Louisville, KY, USA aut NATIONALLICENCE 773 0- Biodegradation Kluwer Academic Publishers 3/2-3(1992-06-01), 137-146 0923-9820 3:2-3<137 1992 3 10532 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer