caa a22 4500 46910595X CHVBK 20180323133056.0 cr unu---uuuuu 170328e19920601xx s 000 0 eng 10.1071/APP9920070 doi (NATIONALLICENCE)springer-10.1071/APP9920070 A rapid shake-elution procedure for quantifying root lesion nematodes ( Pratylenchus thornei ) in chickpea and wheat [Elektronische Daten] [K. Moore, R. Southwell, M. Schwinghamer, R. Murison] A shake-elution procedure was evaluated for quantifying root lesion nematodes (Pratylenchus thornei) in roots of chickpea (Cicer arietinum) and wheat (Triticum aestivum). The procedure was a combination of previously described techniques: shaking root pieces in water, sieving the water to collect the nematodes and centrifuging the nematode sample over a sucrose cushion to remove root debris. The procedure gave very clean samples of P. thornei in which the nematodes were active and readily identifiable. A statistical (failure time) model was used to describe and compare recovery profiles (cumulative recovery over time). The shake-elution procedure recovered nematodes up to 8.3 times faster than a Baermann procedure and up to 2.4 times faster than a misting procedure. For a given experiment, recovery profiles for chickpea and wheat were very similar, even when the total number of nematodes was different by a factor of more than ten. However, recovery rates varied between batches of roots collected at different times. Rate of recovery was not significantly affected by root sample size in the range 1-9 g Australasian Plant Pathology Society, 1992 Moore K. Agricultural Research Centre, New South Wales Agriculture, 2340, Tamworth, New South Wales aut Southwell R. Agricultural Research Centre, New South Wales Agriculture, 2340, Tamworth, New South Wales aut Schwinghamer M. Agricultural Research Centre, New South Wales Agriculture, 2340, Tamworth, New South Wales aut Murison R. Agricultural Research Centre, New South Wales Agriculture, 2340, Tamworth, New South Wales aut Australasian Plant Pathology Springer Netherlands 21/2(1992-06-01), 70-78 0156-0972 21:2<70 1992 21 13313 https://doi.org/10.1071/APP9920070 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1071/APP9920070 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moore K. Agricultural Research Centre, New South Wales Agriculture, 2340, Tamworth, New South Wales aut NATIONALLICENCE 700 1- Southwell R. Agricultural Research Centre, New South Wales Agriculture, 2340, Tamworth, New South Wales aut NATIONALLICENCE 700 1- Schwinghamer M. Agricultural Research Centre, New South Wales Agriculture, 2340, Tamworth, New South Wales aut NATIONALLICENCE 700 1- Murison R. Agricultural Research Centre, New South Wales Agriculture, 2340, Tamworth, New South Wales aut NATIONALLICENCE 773 0- Australasian Plant Pathology Springer Netherlands 21/2(1992-06-01), 70-78 0156-0972 21:2<70 1992 21 13313 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer