caa a22 4500 469109505 CHVBK 20180323133106.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF02039941 doi (NATIONALLICENCE)springer-10.1007/BF02039941 Iodination of placental ferritin for RIA [Elektronische Daten] [A. Glagoličová, V. Wernischová, F. Novák] For purposes of radioimmunoanalytical determination of serum ferritin, conditions for antigen iodination and separation were searched for, which could provide a satisfactory radiochemical purity and specific activity, high immunoreactivity and stability of the resulting labeled product, necessary for an acceptable expiration of the RIA kit. Two iodination methods (chloramine and conjugation methods) were tested, and a three-step procedure was elaborated for iodination and separation by gel column chromatography. The iodinated antigen obtained —125I-placental ferritin with IRmax of about 80%,125I−<8%, specific activity of about 0.6MBq/μg and stability for the expiration period of 3 to 4 months — is quite satisfactory for the RIA applications. Akadémiai Kiadó, 1992 Glagoličová A. Institute of Biophysics and Institute of Nuclear Medicine, 1st Medical Faculty, Charles University, Prague, (ČSFR) aut Wernischová V. Institute of Biophysics and Institute of Nuclear Medicine, 1st Medical Faculty, Charles University, Prague, (ČSFR) aut Novák F. Institute of Biophysics and Institute of Nuclear Medicine, 1st Medical Faculty, Charles University, Prague, (ČSFR) aut Journal of Radioanalytical and Nuclear Chemistry Kluwer Academic Publishers 162/1(1992-09-01), 199-205 0236-5731 162:1<199 1992 162 10967 https://doi.org/10.1007/BF02039941 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02039941 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Glagoličová A. Institute of Biophysics and Institute of Nuclear Medicine, 1st Medical Faculty, Charles University, Prague, (ČSFR) aut NATIONALLICENCE 700 1- Wernischová V. Institute of Biophysics and Institute of Nuclear Medicine, 1st Medical Faculty, Charles University, Prague, (ČSFR) aut NATIONALLICENCE 700 1- Novák F. Institute of Biophysics and Institute of Nuclear Medicine, 1st Medical Faculty, Charles University, Prague, (ČSFR) aut NATIONALLICENCE 773 0- Journal of Radioanalytical and Nuclear Chemistry Kluwer Academic Publishers 162/1(1992-09-01), 199-205 0236-5731 162:1<199 1992 162 10967 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer