caa a22 4500 469109572 CHVBK 20180323133106.0 cr unu---uuuuu 170328e19920901xx s 000 0 eng 10.1007/BF02039919 doi (NATIONALLICENCE)springer-10.1007/BF02039919 High temperature solid state catalytic isotope exchange with deuterium and tritium [Elektronische Daten] [Yu. Zolotarev, V. Kozik, E. Dorokhova, D. Zaitsev, N. Myasoedov, S. Rozenberg] A method for synthesizing tritium- or deuterium-labeled amino acids, peptides and biogenic amines through high temperature solid state catalytic isotope exchange (HSCIE) is proposed. The dependence of the degree of isotope exchange in HSCIE on the structure of the compound, the reactivity of hydrogen at different carbon atoms and the conditions of the process has been examined. If HSCIE is performed in the temperature range of 373 to 413K, the selectivity of isotopic label incorporation comes to 70% or higher. When the tritium label is introduced into peptides, they retain the configuration of asymmetric atoms, even upon the substitution of tritium for hydrogen at the α-carbon atoms of the amino acid residues. HSCIE at 453-513K leads to an even distribution of the isotopic label over the organic compound molecule. The results of3H NMR spectroscopy highlighting the distribution of the tritium label in the organic compound molecules are presented. The configuration of asymmetric atoms in amino acids is preserved to a high extent upon 80-90% substitution of isotopes for hydrogen atoms. Akadémiai Kiadó, 1992 Zolotarev Yu Institute of Molecular Genetics, USSR Academy of Sciences, Moscow, Russia aut Kozik V. Institute of Molecular Genetics, USSR Academy of Sciences, Moscow, Russia aut Dorokhova E. Institute of Molecular Genetics, USSR Academy of Sciences, Moscow, Russia aut Zaitsev D. Institute of Molecular Genetics, USSR Academy of Sciences, Moscow, Russia aut Myasoedov N. Institute of Molecular Genetics, USSR Academy of Sciences, Moscow, Russia aut Rozenberg S. Pharmacology Institute, Academy of Medical Sciences, Moscow, Russia aut Journal of Radioanalytical and Nuclear Chemistry Kluwer Academic Publishers 162/1(1992-09-01), 3-14 0236-5731 162:1<3 1992 162 10967 https://doi.org/10.1007/BF02039919 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02039919 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zolotarev Yu Institute of Molecular Genetics, USSR Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Kozik V. Institute of Molecular Genetics, USSR Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Dorokhova E. Institute of Molecular Genetics, USSR Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Zaitsev D. Institute of Molecular Genetics, USSR Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Myasoedov N. Institute of Molecular Genetics, USSR Academy of Sciences, Moscow, Russia aut NATIONALLICENCE 700 1- Rozenberg S. Pharmacology Institute, Academy of Medical Sciences, Moscow, Russia aut NATIONALLICENCE 773 0- Journal of Radioanalytical and Nuclear Chemistry Kluwer Academic Publishers 162/1(1992-09-01), 3-14 0236-5731 162:1<3 1992 162 10967 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer