caa a22 4500 469110384 CHVBK 20180323133109.0 cr unu---uuuuu 170328e19921101xx s 000 0 eng 10.1007/BF02037491 doi (NATIONALLICENCE)springer-10.1007/BF02037491 Menestrina G. Department of Physics, Faculty of Sciences, University of Trento, Povo, I-38050, Trento, (Italy) aut Ion transport and selectivity in model lipid membranes carrying incorporated cytolytic protein toxins [Elektronische Daten] [G. Menestrina] We used artificial planar lipid membranes to investigate, the mode of action of cytolysins of different origin. We studied some pathologically important bacterial toxins (e. g. S. aureus α-toxin, C. perfringens Θ-toxin, B. thuringiensis δ-endotoxin and E. coli α-hemolysin). All these toxins are used by the bacteria to damage the cells of the invaded organism. We also studied cytolysins of animal origin which are used to react against the attack of foreign organisms like cytolysins from the nematocysts of sea anemones. These proteins disrupt the permeability barrier of the attacked cell membrane by opening a pore into the lipid matrix. We found that in most cases a receptor is not truly required to render them competent to bind to a cell membrane, they spontaneously insert into preformed pure lipid membranes. Several properties of the resulting pores were compared. They are generally large, water filled, and stay open for long periods. In most cases neutral molecules up to a few kDa molecular weight (like sugars and metabolites) can easily pass through the channel. They are weakly selective, usually being able to discriminate only between anions and cations. The selectivity depends on the presence of fixed charges on the protein since it is modulated by pH and by chemical modification of the protein charged residues. Akadémiai Kiadó, 1992 Journal of Radioanalytical and Nuclear Chemistry Kluwer Academic Publishers 163/1(1992-11-01), 169-180 0236-5731 163:1<169 1992 163 10967 https://doi.org/10.1007/BF02037491 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF02037491 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Menestrina G. Department of Physics, Faculty of Sciences, University of Trento, Povo, I-38050, Trento, (Italy) aut NATIONALLICENCE 773 0- Journal of Radioanalytical and Nuclear Chemistry Kluwer Academic Publishers 163/1(1992-11-01), 169-180 0236-5731 163:1<169 1992 163 10967 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer