caa a22 4500 469114916 CHVBK 20180323133122.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00731173 doi (NATIONALLICENCE)springer-10.1007/BF00731173 Enzyme-linked immunosorbent assays for the measurement of blood group A and B glycosyltransferase activities [Elektronische Daten] [Lakhu Keshvara, Elizabeth Newton, A. Good, Ole Hindsgaul, Monica Palcic] ELISA assays have been developed for α(1-3)N-acetylgalactosaminyltransferase (blood group A transferase) and α(1-3)galactosyltransferase (blood group B transferase) activities. In these assays, microtitre plates coated with the bovine serum albumin conjugate of a synthetic Fucα1-2Galβ-R acceptor substrate are incubated with the appropriate nucleotide donor (UDP-GalNAc or UDP-Gal) and human serum as the enzyme source. The resulting trisaccharide products Fucα1-2(GalNAcα1-3)Galβ-R-BSA or Fucα1-2(Galα1-3)Galβ-R-BSA are detected and quantified with monoclonal antibodies selected not to cross-react with the substrate structure. With less than a microliter of human serum, product formation is proportional to enzyme concentration and to time of incubation of up to 90 min. Chapman and Hall, 1992 ELISA nationallicence blood group A N -acetylgalactosaminyltransferase nationallicence blood group B galactosyltransferase nationallicence glycosyltransferase assays nationallicence Keshvara Lakhu Department of Food Science, University of Alberta, T6G 2P5, Edmonton, Alberta, Canada aut Newton Elizabeth Department of Food Science, University of Alberta, T6G 2P5, Edmonton, Alberta, Canada aut Good A. Chembiomed Ltd., Edmonton Research and Development Park, Station F, P.O. Box 8050, T6H 4N9, Edmonton, Alberta, Canada aut Hindsgaul Ole Department of Chemistry, University of Alberta, T6G 2G2, Edmonton, Alberta, Canada aut Palcic Monica Department of Food Science, University of Alberta, T6G 2P5, Edmonton, Alberta, Canada aut Glycoconjugate Journal Kluwer Academic Publishers 9/1(1992-02-01), 16-20 0282-0080 9:1<16 1992 9 10719 https://doi.org/10.1007/BF00731173 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731173 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Keshvara Lakhu Department of Food Science, University of Alberta, T6G 2P5, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Newton Elizabeth Department of Food Science, University of Alberta, T6G 2P5, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Good A. Chembiomed Ltd., Edmonton Research and Development Park, Station F, P.O. Box 8050, T6H 4N9, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Hindsgaul Ole Department of Chemistry, University of Alberta, T6G 2G2, Edmonton, Alberta, Canada aut NATIONALLICENCE 700 1- Palcic Monica Department of Food Science, University of Alberta, T6G 2P5, Edmonton, Alberta, Canada aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/1(1992-02-01), 16-20 0282-0080 9:1<16 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer