caa a22 4500 469114924 CHVBK 20180323133122.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00731174 doi (NATIONALLICENCE)springer-10.1007/BF00731174 Detection of the ganglioside N -glycolyl-neuraminyl-lactosyl-ceramide by biotinylated Escherichia coli K99 lectin [Elektronische Daten] [Abdelouahab Ouadia, Yannis Karamanos, Raymond Julien] K99 lectin fromEscherichia coli was purified and biotinylated via its carboxyl groups using biocytin hydrazide and a water soluble carbodiimide. Biotinylation of two out of the nine carboxyl groups was sufficient to permit detection of the lectin by avidin and did not cause any loss of the haemagglutinating activity. It was demonstrated that the biotinylated K99 lectin retained other important properties of native K99 and that it will probably become a very sensitive detecting reagent. Indeed, it was able to bind to HeLa cells, as do intact bacteria carrying K99 fimbriae, and also to recognizeN-glycolyl-neuraminyl-lactosyl-ceramide in an overlay binding assay. Chapman and Hall, 1992 K99 lectin nationallicence NeuGe-GM3 detection nationallicence Ouadia Abdelouahab Institut de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges cedex, France aut Karamanos Yannis Institut de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges cedex, France aut Julien Raymond Institut de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges cedex, France aut Glycoconjugate Journal Kluwer Academic Publishers 9/1(1992-02-01), 21-26 0282-0080 9:1<21 1992 9 10719 https://doi.org/10.1007/BF00731174 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731174 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ouadia Abdelouahab Institut de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges cedex, France aut NATIONALLICENCE 700 1- Karamanos Yannis Institut de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges cedex, France aut NATIONALLICENCE 700 1- Julien Raymond Institut de Biotechnologie, Université de Limoges, 123, avenue Albert Thomas, 87060, Limoges cedex, France aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/1(1992-02-01), 21-26 0282-0080 9:1<21 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer