caa a22 4500 469114932 CHVBK 20180323133122.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00731178 doi (NATIONALLICENCE)springer-10.1007/BF00731178 Binding receptors for α- l -fucosidase in human B-lymphoid cell lines [Elektronische Daten] [Richard Dicioccio, Arnold Miller] An established mechanism for directing newly made acid hydrolases to lysosomes involves acquisition of mannose 6-phosphate residues by the carbohydrate portion of acid hydrolases followed by binding to specific membrane-bound transport receptors and delivery to lysosomes. Two distinct phosphomannosyl receptors (CI-MPR and CD-MPR) have been identified. Alternative mechanisms for trafficking acid hydrolases exist. This report examines means for the possible receptor-mediated intracellular transport of α-l-fucosidase in lymphoid cells. The binding of α-l-fucosidase to intact cells and to total cell membrane preparations, in conjunction with immunoassays of solubilized membrane preparations, revealed the presence of CI-MPR and CD-MPR on human lymphoid and fibroblast cell lines. The mean level of CD-MPR in nine lymphoid cell lines was 7.2-fold greater than CI-MPR. The mean level of CI-MPR in two fibroblast lines was 3.8-fold greater than CD-MPR. The mean content of CI-MPR was 19.5-fold greater in the fibroblasts than in the lymphoid cells. The CD-MPR content of fibroblasts and lymphoid cells was nearly equivalent. Among these cell lines were a fibroblast and a lymphoid line from the same individual. These results indicate that human B-lymphoid cells are deficient in CI-MPR and suggest that modulation of expression of CI-MPR and CD-MPR in lymphoid cells differs from that in fibroblasts, including cell lines with identical genomes. No specific receptor capable of binding α-l-fucosidase independent of mannose 6-phosphate was demonstrable, despite published results that support the existence of a mannose 6-phosphate independent trafficking mechanism in lymphoid cells for this enzyme. Chapman and Hall, 1992 α- l -Fucosidase nationallicence phosphomannosyl receptors nationallicence B-lymphoid cells nationallicence Dicioccio Richard Department of Gynaecologic Oncology, Roswell Park Cancer Institute, 14263, Buffalo, NY, USA aut Miller Arnold Department of Neurosciences, M-024, University of California, San Diego, School of Medicine, 90093, La Jolla, CA, USA aut Glycoconjugate Journal Kluwer Academic Publishers 9/1(1992-02-01), 56-62 0282-0080 9:1<56 1992 9 10719 https://doi.org/10.1007/BF00731178 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731178 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dicioccio Richard Department of Gynaecologic Oncology, Roswell Park Cancer Institute, 14263, Buffalo, NY, USA aut NATIONALLICENCE 700 1- Miller Arnold Department of Neurosciences, M-024, University of California, San Diego, School of Medicine, 90093, La Jolla, CA, USA aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/1(1992-02-01), 56-62 0282-0080 9:1<56 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer