caa a22 4500 469114940 CHVBK 20180323133122.0 cr unu---uuuuu 170328e19920201xx s 000 0 eng 10.1007/BF00731175 doi (NATIONALLICENCE)springer-10.1007/BF00731175 Production and characterization of a monoclonal antibody (BBH5) directed to ganglioside lactone [Elektronische Daten] [Bernadette Bouchon, Steven Levery, Henrik Clausen, Sen-Itiroh Hakomori] The occurrence of lactones in various ganglioside preparations has been clearly demonstrated, yet the natural occurrence of ganglioside lactones in cells and tissues has been the subject of long debate, since lactones can be formed readily during preparation of gangliosides. We now report the generation of a monoclonal antibody (BBH5) that reacts specifically with lactones of disialogangliosides having the NeuAc2-8NeuAc2-3Gal sequence, but does not crossreact with the parent ganglioside. The specificity of the antibody resides on the first lactone ring between two sialic acid residues but not on the second lactone ring between sialic acid and galactose, as evidenced by reactivity with lactonized GD1b having the first lactone ring (L1), and by reactivity with lactonized polysialic acid homo-oligomers ([NeuAcα2-8] n NeuAc). The sialic acid carboxyl involved in the lactone ring was unequivocally determined after ammonolysis followed by methylation and fast atom bombardment mass spectrometry. The antibody BBH5 thus provides a novel tool for studies of the natural occurrence of lactones in cells and tissues. Chapman and Hall, 1992 Lactone nationallicence ganglioside nationallicence monoclonal antibody nationallicence sialic acid nationallicence BSA : bovine serum albumin nationallicence CM : chloroform-methanol nationallicence CMW : chloroform-methanol-water nationallicence FAB-MS : fast atom bombardment mass spectrometry nationallicence IHW : isopropanol-hexane-water nationallicence MAb : monoclonal antibody nationallicence PBS : phosphate-buffered saline nationallicence TLC : thin layer chromatography nationallicence Bouchon Bernadette The Biomembrane Institute, 201 Elliott Ave W, 98119, Seattle, WA, USA aut Levery Steven The Biomembrane Institute, 201 Elliott Ave W, 98119, Seattle, WA, USA aut Clausen Henrik The Biomembrane Institute, 201 Elliott Ave W, 98119, Seattle, WA, USA aut Hakomori Sen-Itiroh The Biomembrane Institute, 201 Elliott Ave W, 98119, Seattle, WA, USA aut Glycoconjugate Journal Kluwer Academic Publishers 9/1(1992-02-01), 27-38 0282-0080 9:1<27 1992 9 10719 https://doi.org/10.1007/BF00731175 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/BF00731175 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bouchon Bernadette The Biomembrane Institute, 201 Elliott Ave W, 98119, Seattle, WA, USA aut NATIONALLICENCE 700 1- Levery Steven The Biomembrane Institute, 201 Elliott Ave W, 98119, Seattle, WA, USA aut NATIONALLICENCE 700 1- Clausen Henrik The Biomembrane Institute, 201 Elliott Ave W, 98119, Seattle, WA, USA aut NATIONALLICENCE 700 1- Hakomori Sen-Itiroh The Biomembrane Institute, 201 Elliott Ave W, 98119, Seattle, WA, USA aut NATIONALLICENCE 773 0- Glycoconjugate Journal Kluwer Academic Publishers 9/1(1992-02-01), 27-38 0282-0080 9:1<27 1992 9 10719 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer